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首页> 外文会议>Conference on Multiphoton Microscopy in the Biomedical Sciences >The LSM 5 family: An integrated imaging and spectroscopic platform for the study of cellular dynamic processes
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The LSM 5 family: An integrated imaging and spectroscopic platform for the study of cellular dynamic processes

机译：LSM 5系列：用于研究蜂窝动态过程的综合成像和光谱平台

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The elucidation of diffusion processes and molecular interactions and their relation to compartments and structures will be essential to understand cellular functions in detail. Often it is not the average signal that is of interest but the behaviour of single molecules which behave as individuals. Fluorescence based assays have revolutionized the way we can observe molecules at work in their natural cellular settings and they have now also become available for single molecule studies. These technologies comprise Fluorescence Fluctuation Analysis (FFA) including Fluorescence Correlation Spectroscopy (FCS), Fluorescence Redistribution After Photobleaching (FRAP), Foerster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging (FLIM). Especially for dual colour experiments and when dealing with delicate samples the employment of multiphoton microscopy using the aforementioned technologies can be of great benefit. Ideal instruments to study single molecules would therefore need to accommodate equipment that allow for fast time resolution, adequate detectors and lasers as well as integrated work flows. In this contribution we discuss the newest developments in commercial instrumentation and software at Carl Zeiss towards highly sensitive imaging in combination with spectroscopic analysis.
机译：扩散方法和分子相互作用的阐明及其与隔室和结构的关系对于了解细胞功能将详细阐述至关重要。通常它不是感兴趣的平均信号，而是表现为个人的单一分子的行为。荧光基测定彻底改变了我们可以观察到其天然细胞环境中的分子的方式，并且现在也可用于单分子研究。这些技术包括包括荧光相关光谱（FCS）的荧​​光波动分析（FFA），光漂白后（FRAP）后的荧光再分布，Foerster谐振能量转移（FRET）和荧光寿命成像（FLIM）。特别是对于双重颜色实验以及处理精致的样本时，使用上述技术的多选显微镜就业可能具有很大的好处。因此，学习单一分子的理想仪器需要容纳允许快速时间分辨率，适当探测器和激光以及集成工作流程的设备。在这一贡献中，我们讨论了Carl Zeiss对高度敏感成像的商业仪表和软件的最新发展，与光谱分析相结合。

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