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首页> 外文会议>Multiphoton Microscopy in the Biomedical Sciences VI; Progress in Biomedical Optics and Imaging; vol.7 no.12 >The LSM 5 family: An integrated imaging and spectroscopic platform for the study of cellular dynamic processes
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The LSM 5 family: An integrated imaging and spectroscopic platform for the study of cellular dynamic processes

机译：LSM 5系列：用于细胞动态过程研究的集成成像和光谱平台

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摘要

The elucidation of diffusion processes and molecular interactions and their relation to compartments and structures will be essential to understand cellular functions in detail. Often it is not the average signal that is of interest but the behaviour of single molecules which behave as individuals. Fluorescence based assays have revolutionized the way we can observe molecules at work in their natural cellular settings and they have now also become available for single molecule studies. These technologies comprise Fluorescence Fluctuation Analysis (FFA) including Fluorescence Correlation Spectroscopy (FCS), Fluorescence Redistribution After Photobleaching (FRAP), Foerster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging (FLIM). Especially for dual colour experiments and when dealing with delicate samples the employment of multiphoton microscopy using the aforementioned technologies can be of great benefit. Ideal instruments to study single molecules would therefore need to accommodate equipment that allow for fast time resolution, adequate detectors and lasers as well as integrated work flows. In this contribution we discuss the newest developments in commercial instrumentation and software at Carl Zeiss towards highly sensitive imaging in combination with spectroscopic analysis.
机译：阐明扩散过程和分子相互作用及其与区室和结构的关系对于详细了解细胞功能至关重要。通常，感兴趣的不是平均信号，而是表现为个体的单个分子的行为。基于荧光的测定法彻底改变了我们观察分子在其天然细胞环境中工作的方式，现在它们也可用于单分子研究。这些技术包括荧光波动分析（FFA），包括荧光相关光谱（FCS），光漂白后的荧光再分布（FRAP），福斯特共振能量转移（FRET）和荧光寿命成像（FLIM）。尤其是对于双色实验，以及在处理精密样品时，使用上述技术的多光子显微镜检查可能会带来极大的好处。因此，研究单个分子的理想仪器需要配备能够快速分辨时间的设备，足够的检测器和激光器以及集成的工作流程。在本文中，我们讨论了卡尔·蔡司商用仪器和软件的最新发展，这些研究朝着结合光谱分析的高灵敏度成像发展。

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