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首页> 外文会议>Conference on Multiphoton Microscopy in the Biomedical Sciences >Two-photon spectral imaging microscopy of skin tissues
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Two-photon spectral imaging microscopy of skin tissues

机译：皮肤组织的双光子光谱成像显微镜

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The last two decades saw the emergence of spectroscopy and microscopic imaging as techniques for tissue diagnostics. The biochemical state of the tissue is revealed by spectroscopy, while the morphological information is visualized by microscopic imaging. Little research has been carried out to diagnose tissues based on the combination of spectroscopy and microscopic imaging. Here, we report on tissue spectroscopy and microscopic imaging employing two-photon excitation of tissue autofluorescence and second harmonic generation. We designed and constructed a prism-based spectral imaging system coupled to a two-photon microscope. Full emission spectra with a 1-7 nm spectral resolution covering 330nm to 600nm can be recorded at a maximum rate of 500 spectra per second equivalent to about 0.5 frames/min (224x224 pixels). We present results on spectral imaging of human skin sections and in-depth imaging of pig skin tissue. Different skin layers show clear differences in their intrinsic emission spectral signature that can be used for diagnosis.
机译：过去二十年来表现出光谱和微观成像作为组织诊断的技术。通过光谱揭示组织的生物化学状态，而通过微观成像，形态学信息被视为形态学信息。已经基于光谱学和微观成像的组合来进行少研究以诊断组织。在此，我们报告了组织光谱和微观成像，采用两光荧光和二次谐波产生的双光子激发。我们设计并构建了基于棱镜的光谱成像系统，耦合到双光子显微镜。具有覆盖330nm至600nm的具有1-7nm光谱分辨率的完全发射光谱可以以每秒500个光谱的最大速率相当于约0.5帧/分钟（224x224像素）。我们呈现了人体皮肤部分的光谱成像和猪皮肤组织的深入成像的结果。不同的皮肤层显示出可用于诊断的内在发射光谱特征的明显差异。

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