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首页> 外文会议>Multiphoton Microscopy in the Biomedical Sciences VI; Progress in Biomedical Optics and Imaging; vol.7 no.12 >Two-photon spectral imaging microscopy of skin tissues
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Two-photon spectral imaging microscopy of skin tissues

机译：皮肤组织的双光子光谱成像显微镜

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摘要

The last two decades saw the emergence of spectroscopy and microscopic imaging as techniques for tissue diagnostics. The biochemical state of the tissue is revealed by spectroscopy, while the morphological information is visualized by microscopic imaging. Little research has been carried out to diagnose tissues based on the combination of spectroscopy and microscopic imaging. Here, we report on tissue spectroscopy and microscopic imaging employing two-photon excitation of tissue autofluorescence and second harmonic generation. We designed and constructed a prism-based spectral imaging system coupled to a two-photon microscope. Full emission spectra with a 1-7 nm spectral resolution covering 330nm to 600nm can be recorded at a maximum rate of 500 spectra per second equivalent to about 0.5 frames/min (224x224 pixels). We present results on spectral imaging of human skin sections and in-depth imaging of pig skin tissue. Different skin layers show clear differences in their intrinsic emission spectral signature that can be used for diagnosis.
机译：在过去的二十年中，光谱学和显微成像技术作为组织诊断技术的出现。通过光谱显示组织的生化状态，同时通过显微成像观察形态信息。基于光谱学和显微成像相结合的诊断组织的研究很少。在这里，我们报告组织光谱学和显微成像采用组织自发荧光和二次谐波产生的双光子激发。我们设计并构建了一个基于棱镜的光谱成像系统，该系统与双光子显微镜耦合。可以以每秒500个光谱的最大速率（相当于约0.5帧/分钟（224x224像素））记录具有1-7 nm光谱分辨率（覆盖330nm至600nm）的全发射光谱。我们目前在人类皮肤切片的光谱成像和猪皮肤组织的深度成像方面的研究成果。不同的皮肤层在可用于诊断的固有发射光谱特征上显示出明显的差异。

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