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Non-radioactive determination of phosphoamino acids in peptides and proteins by capillary electrophoresis

机译：毛细管电泳肽和蛋白质中磷酸氨基酸的非放射性测定

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A new method is described that allows non-radioactive determination of all phosphoamino acids in peptides or proteins. It involves partial hydrolysis of peptides or proteins using 6 N HCl at 110 deg C for 1.5 hour, then derivatization of the liberated amino acids with phenylisothiocyanate and finally separation of all PTH-phosphoamino acids from other PTH-amino acids or peptides by capillary electrophoresis. The detection limits are 170 fmol for phosphotyrosine, 350 fmol for phosphothreonine and 700 fmol for phosphoserine.

机译：描述了一种新方法，其允许肽或蛋白质中的所有磷酸氨基酸的非放射性测定。它涉及在110℃下使用6nHCl的肽或蛋白质的部分水解1.5小时，然后用苯乙酸苯二氰酸酯衍生释放的氨基酸，并通过毛细管电泳将所有Pth-磷酸氨基酸的所有Pth-磷酸氨基酸分离。检测限为磷酸酚酶，350 fmol的170 fmol，磷酸胆碱的350 fmol，磷源为700 fmol。

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《Chinese peptide symposium》|1998年||共3页
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F.-Y.Che; X.-X.Shao; L.-G.Xu; R.Zeng; Q.-C.Xia;
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中图分类抗体蛋白与干扰素蛋白;
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1. Rapid and sensitive determination of phosphoamino acids in phosvitin by N-hydroxysuccinimidyl fluorescein-O-acetate derivatization and capillary zone electrophoresis with laser-induced fluorescence detection [J] . Deng YH, Zhang HS, Wang H Analytical and bioanalytical chemistry . 2008,第1a2期

机译：N-羟基琥珀酰亚胺基荧光素-O-乙酸酯衍生化和毛细管区带电泳-激光诱导荧光检测法快速，灵敏地测定磷脂酰肌醇中的磷酸氨基酸
2. Rapid and sensitive determination of phosphoamino acids in phosvitin by N-hydroxysuccinimidyl fluorescein-O-acetate derivatization and capillary zone electrophoresis with laser-induced fluorescence detection [J] . Ying-Hua Deng, Hua-Shan Zhang, Hong Wang Analytical and Bioanalytical Chemistry . 2008,第1a2期

机译：N-羟基琥珀酰亚胺基荧光素-O-乙酸酯衍生化和毛细管区带电泳-激光诱导荧光检测法快速，灵敏地测定磷脂酰肌醇中的磷酸氨基酸
3. Single-step analysis of low abundance phosphoamino acids via on-line sample preconcentration with chemical derivatization by capillary electrophoresis [J] . Ptolemy AS, Britz-McKibbin P The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry . 2005,第9期

机译：通过在线样品预富集和毛细管电泳化学衍生法对低丰度磷酸氨基酸进行单步分析
4. Non-radioactive determination of phosphoamino acids in peptides and proteins by capillary electrophoresis [C] . F.-Y.Che, X.-X.Shao, L.-G.Xu, Chinese peptide symposium . 1998

机译：毛细管电泳肽和蛋白质中磷酸氨基酸的非放射性测定
5. Assay for detection of mtDNA polymorphisms using peptide nucleic acids and capillary electrophoresis with laser-induced fluorescence. [D] . Pyatski, Yelena. 1999

机译：使用肽核酸和激光电泳的毛细管电泳检测mtDNA多态性的检测方法。
6. Single Shot Capillary Zone Electrophoresis-Mass Spectrometry Produces Over 27000 Peptide and nearly 4400 Protein Identifications by Combining a Very Low-Electroosmosis Coated Capillary a Third-Generation Electrokinetically-Pumped Sheath-Flow Nanospray Interface an Orbitrap Fusion Lumos Tribid Mass Spectrometer and an Advanced Peak Determination Algorithm [O] . Zhenbin Zhang, Alexander S. Hebert, Michael S. Westphall, -1

机译：单发毛细管区带电泳质谱通过结合极低电渗涂膜的毛细管第三代电动泵鞘流纳米喷雾接口Orbitrap Fusion Lumos Tribid质谱仪和一个质谱仪可产生超过27000个肽和近4400个蛋白质鉴定先进的峰确定算法
7. Production of Over 27 000 Peptide and Nearly 4400 Protein Identifications by Single-Shot Capillary-Zone Electrophoresis–Mass Spectrometry via Combination of a Very-Low-Electroosmosis Coated Capillary, a Third-Generation Electrokinetically-Pumped Sheath-Flow Nanospray Interface, an Orbitrap Fusion Lumos Tribrid Mass Spectrometer, and an Advanced-Peak-Determination Algorithm [O] . Zhenbin Zhang, Alexander S. Hebert, Michael S. Westphall, 2018

机译：通过单次毛细管区电泳 - 质谱法通过组合产生超过27 000份肽和近4400蛋白鉴定，通过非常低电渗涂层毛细管，第三代电动泵浦鞘流纳米普通接口，围绕熔化融合Lumos Tribrid质谱仪和先进峰值测定算法

Non-radioactive determination of phosphoamino acids in peptides and proteins by capillary electrophoresis

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