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首页> 外文会议>Annual Meeting of the Florida State Horticultural Society >Improved Removal of Ascorbate Interference in the Folin-Ciocalteu Assay of 'Total Phenolic Content'
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Improved Removal of Ascorbate Interference in the Folin-Ciocalteu Assay of 'Total Phenolic Content'

机译：改善了“总酚类含量”的Folin-Ciocalteu测定中的抗坏血酸干扰

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The venerable Folin-Ciocalteu (F-C) assay for total phenolics can have severe limitations due to interference by ascorbic acid (AsA). For common fruit juices AsA interference can substantially exceed the magnitude of the total phenolic signal. Ascorbate oxidase (AO) has been a promising approach to eliminating the AsA interference, but the oxidation product of AsA by AO is dehydroascorbic acid (DHA), which is an F-C reductant that gives an apparent signal implying a molar strength of 15% to 30% of the original AsA. Hydrogen peroxide (H_2O_2) was successfully used to degrade this residual DHA into products that are not F-C reductants. Using a model orange juice, the impact of this small amount of (-450 ppm) of H_2O_2 on the orange and non-orange phenolic measurements was examined. Chemical means were sought to quench the H_2O_2 oxidative activities shortly after it destroyed the DHA, or to otherwise prevent interaction with the phenolics present without altering the F-C signal. Some promising candidates were identified, but none protected the phenolics completely. The best that can be said is that conditions can be easily set so that the alteration in phenolics represents less than a 10% perturbation in the untreated model juice F-C signal. Although this is much better than the large errors originally seen from AsA (100% or more of a typical orange juice phenolic signal) or from the residual DHA (15% to 30% error), further work is needed to develop an approach to the point that it predictably causes no more than a 5% artifactual error.
机译：由于抗坏血酸（ASA）的干扰，总酚类的古老叶子 - Ciocalteu（F-C）测定可能具有严重的限制。对于常见的果汁汁，ASA干扰可以基本上超过总酚类信号的大小。抗坏血酸氧化酶（AO）是消除ASA干扰的有希望的方法，但AO的ASA氧化产物是脱氢血脂酸（DHA），其是FC还原剂，其表达信号暗示摩尔强度为15％至30的摩尔强度原始ASA的百分比。过氧化氢（H_2O_2）成功地用于将该残留的DHA降解到不是F-C还原剂的产品中。使用橙汁橙汁，检查了少量（-450ppm）的H_2O_2对橙色和非橙色酚醛测量的影响。试图在破坏DHA后不久的情况下终止化学手段，或者以其他方式防止与存在的酚类的相互作用而不改变F-C信号。一些有希望的候选人被确定，但没有完全保护酚类。可以说的最好的是，可以容易地设置条件，使得酚类的变化在未处理的模型果汁F-C信号中表示扰动小于10％。虽然这比最初从ASA（典型橙汁酚类信号的100％或更多）或残留的DHA（15％到30％错误）的大误差要好得多，但是需要进一步的工作来发展一种方法指出它可预测地导致不超过5％的艺术误差。

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