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首页> 外文会议>Beltwide Cotton Conference >PROFILING SEASONAL EXPRESSION OF CRY2AB IN BOLLS OF DUAL-TOXIN BT COTTON
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PROFILING SEASONAL EXPRESSION OF CRY2AB IN BOLLS OF DUAL-TOXIN BT COTTON

机译：在双毒素BT棉棉铃中剖析Cry2ab的季节表达

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Laboratory studies were conducted to quantify toxin levels in bolls located at various locations throughout dual-toxin Bt cottonplants. Results of ELISA techniques indicate that there was a significant quadratic relationship between node locationand Cry2Ab concentration, with expression levels highest in bolls from the central portion of the plant. For bolls at node 9and above (averaged over positions 1 and 2), there was a significant decline in Cry2 expression as the season progressed. Amore dramatic reduction of Cry2Ab levels was noted above node 16 in bolls from position 1and node 14 in bolls from position2. Since bolls from these locations are from the same cohort (i.e., the same phenological age), this would indicate thatenvironmental factors may play a significant role in expression of Bt toxins throughout the plant.
机译：进行实验室研究以量化位于各种位置的棉铃中的毒素水平，其各个地点在两种毒素BT棉花。 ELISA技术的结果表明，节点位置和Cry2ab浓度之间存在显着的二次关系，表达水平来自植物中央部分的棉铃中最高。对于节点9的棉铃（在上面平均1和2），随着赛季的进展，Cry2表达的显着下降。在来自位置2的棉铃中的位置1和节点14的棉铃中的节点16上方，在棉铃上的节点16上方注意到Cry2ab水平的浑浊减少。由于来自这些位置的棉铃，因此来自同一队列（即，相同的酚类年龄），这表明环境中的因素可能在整个植物中表达Bt毒素的表达中发挥着重要作用。

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