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首页> 外文会议>Beltwide Cotton Conference >CROSS-RESISTANCE LEVELS BETWEEN THE BACILLUS THURINGIENSIS PROTEINS CRY1AC AND VIP3A IN HELIOTHIS VIRESCENS
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CROSS-RESISTANCE LEVELS BETWEEN THE BACILLUS THURINGIENSIS PROTEINS CRY1AC AND VIP3A IN HELIOTHIS VIRESCENS

机译：在Heliothis virescens的芽孢杆菌蛋白蛋白蛋白Cry1ac和Vip3a之间的交叉抵抗水平

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Laboratory experiments were conducted with four strains of tobacco budworm, Heliothis virescens (F.), to determine the level of cross-resistance between the Bacillus thuringiensis toxins cry1Ac and vip3A using concentration-mortality experiments. H. virescens strains consisted of three cry1Ac-resistant lines (YHD2, KCBhyb, and CXC) and one susceptible line (YDK). Concentration-mortality data demonstrated that the three Cry1Ac-resistant H. virescens strains were at least 52- to 424-fold resistant to cry1Ac compared to the susceptible strain. Concentration-mortality tests with vip3A demonstrated that mortality was similar among all four H. virescens strains. Relative larval growth on cry1Ac reflected concentration-mortality test results. Relative larval growth on vip3A was variable at lower doses but was more consistent on concentrations above 25 μg/ml. These results demonstrate that cross-resistance did not exist between cry1Ac and vip3A in H. virescens. Therefore, the introduction of VipCot linescould delay cry1Ac-resistance evolution in H. virescens.
机译：实验室实验用四种烟草芽虫，Heliothis Virescens（F.）进行，以确定使用浓度 - 死亡率实验的芽孢杆菌毒素毒素Cry1Ac和VIP3a之间的交叉抗性水平。 H. Virescens菌株由三条抗性线（YHD2，KCBHYB和CXC）和一个敏感线（YDK）组成。浓度 - 死亡率数据表明，与易感菌株相比，抗静脉抗性H. virescens菌株的抗性至少为52至424倍。用VIP3A浓度 - 死亡率试验证明，所有四个H.Vivescens菌株中的死亡率相似。 Cry1AC对Cry1AC的相对幼虫生长反映了浓度 - 死亡率测试结果。 VIP3a上的相对幼虫生长在较低剂量下变化，但在25μg/ ml以上的浓度上更一致。这些结果表明，在H.Vivescens的Cry1Ac和VIP3a之间不存在交叉抗性。因此，引入VIPCOT线可以延迟H.Virescens的Cry1Ac抵抗力进化。

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