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MOLECULAR BIOLOGICAL TECHNIQUES FOR SUBSPECIES IDENTIFICATION: AN OVERVIEW

机译:用于亚种的分子生物学技术识别:概述

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Amongst complementary approaches, structural identification of pathogens and toxins is a privileged strategy of analysis of suspicious outbreaks. Molecular tools can be used to examine both the outer antigenic surface of the pathogen and its genome. The major assets of these molecular tools lie in their extreme sensitivity and the short time they require to provide clues or answers. Another advantage is their ability to perform on pathogen fragments, dead viruses or bacteria, denatured toxins, or even fossils of pathogens that have been dead for years. Identification requires previous knowledge that may range from databases of antigenic structures or serotype-specific antibodies to genome databases. For each tool, the molecular signature it provides for the sample needs to be compared to a set of standards. Assembling and validating such standards is a crucial aspect of the whole strategy in the perspective of monitoring BTW proliferation.
机译:在互补方法中,病原体和毒素的结构鉴定是一种特权分析可疑爆发的策略。分子工具可用于检查病原体的外抗原表面及其基因组。这些分子工具的主要资产呈现出极端敏感性和提供线索或答案所需的短时间。另一个优点是它们能够在病原体片段,死病毒或细菌,变性毒素,甚至多年来已经死亡的病原体的化石。鉴定需要先前的知识,其可以从抗原结构或血清型特异性抗体的数据库到基因组数据库。对于每个工具,需要将样本提供的分子签名与一组标准进行比较。在监测BTW增殖的角度来看,组装和验证此类标准是整个策略的关键方面。

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