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首页> 外文会议>AIChE Annual Meeting >Impedance Array Studies of Mammalian Cell Growth
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Impedance Array Studies of Mammalian Cell Growth

机译：哺乳动物细胞生长的阻抗阵列研究

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Functional genomic studies and drug candidate testing both require high throughput, parallel experimentation to screen for variable cellular behaviors. Here we present the use of impedance sensing electrode array where each site is comparable to the area spanned by a few cells. The electrical signal from each sensing site provides information not only on cell “presence,” but also on the extent of cell attachment to the substrate. Data will be presented on the signals provided by mouse fibroblasts growing on 9 similar sized electrodes. In the absence of cells, each electrode’s impedance was found to depend as expected on electrode size and frequency. More notably, the impedance increased up to four-fold when fibroblasts attached and spread out over time. These findings suggest that such impedance sensors can detect cell “presence” as well as the extent of cell attachment to the substrate. Finite element simulation shows a good agreement with experimental results. From a technological perspective, impedance sensors could play a role in automating cell proliferation assays. Alternately, in structures with many sensors and sparse cell coverage, cell tracking and motility experiments may be potentially conducted. Finally, it may be possible to use such impedance measurements to screen mutants and malignant cells as well as effectors for altered adhesion prior to employing more demanding methods.
机译：功能基因组研究和药物候选测试两者都需要高通量，并行实验到可变蜂窝行为的筛选。在这里，我们介绍了使用阻抗感测电极阵列，其中每个部位与几个细胞跨越的区域相当。来自每个感测场地的电信号不仅提供了电池“存在”的信息，而且还提供了对基板的电池附着的程度。数据将呈现在由9个相似尺寸电极上生长的小鼠成纤维细胞提供的信号。在没有细胞的情况下，发现每个电极的阻抗在电极尺寸和频率上随着预期的预期取决于预期。更值得注意的是，当成纤维细胞附着并随时间展开时，阻抗增加到四倍。这些发现表明，这种阻抗传感器可以检测细胞“存在”以及对基板的电池附着的程度。有限元模拟显示了与实验结果的良好一致性。从技术角度来看，阻抗传感器可以在自动化细胞增殖测定中发挥作用。或者，在具有许多传感器和稀疏单元覆盖的结构中，可以潜在地进行细胞跟踪和运动实验。最后，可以使用这种阻抗测量来筛选突变体和恶性细胞以及在采用更苛刻的方法之前用于改变粘合的效果。

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