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首页> 外文会议>Annual Meeting of the American Association of Cereal Chemists International >Development of a 10 g corn wet-milling procedure and comparison with 100 g procedures
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Development of a 10 g corn wet-milling procedure and comparison with 100 g procedures

机译：开发10g玉米湿铣过程并与100克手术进行比较

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A very small scale wet-milling procedure (~10g) is necessary to test wet-milling characteristics of corn in early stages of corn hybrid development and would be useful to corn breeding programs. We adapted 100g laboratory wet-milling methods already widely used to evaluate extractable starch to measure wet-milling properties with as little as 10g of corn. We selected three corn hybrids with known differences in wet-milling properties and compared them using three procedures: a. the standard 100g procedure developed at University of Illinois; b. a modified 100g procedure; and c. our 10g experimental procedure. Although interactions were found between Procedure and Hybrid factors, fraction yields (except for germ) of the three hybrids were ranked thesame with the three procedures. Germ separation was different for the three procedures. Flotation and screening methods were likely affected by the hybrid germ density and germ size. However, hand-picking the germ in the 10g procedure was efficient in recovering a pure germ fraction. The two 100g procedures performed very similarly except for fiber recovery. The modified 100g procedure was more efficient in recovering fibers by using screens and intensive washing. The 10g procedure was able to rank thehybrids in the same order as the two 100g procedures in terms of extractable starch yield; however, hybrid effects on the starch/gluten separation were more pronounced. Less starch of less purity was recovered for the hybrid with the lowest extractable starch. The 10g procedure will be beneficial to measuring milling efficiency of early generations of corn hybrids where limited samples are available, such as when valuable recombinant proteins are expressed.
机译：非常小的湿磨程序（〜10g）是测试玉米杂交发育早期阶段的玉米湿磨特特征，对玉米育种计划有用。我们改编了100G实验室湿铣方法，已经广泛用于评估萃取的淀粉，以测量湿磨性，只需10g玉米。我们选择了三种玉米杂交物，具有已知湿磨性的差异，并使用三个程序进行比较：a。伊利诺伊大学开发的标准100G程序;湾修改的100g程序;和c。我们的10G实验程序。虽然在程序和杂交因子之间发现相互作用，但三个杂种的级分产率（胚芽除外）被排名为三个程序。对于三个程序，胚芽分离不同。浮选和筛选方法可能受杂交胚芽密度和细菌尺寸的影响。然而，在10G过程中挑选胚芽的胚芽在回收纯细菌级分中是有效的。除光纤恢复外，两个100G程序非常相似。通过使用屏幕和密集的洗涤，改性的100G步骤更有效地回收纤维。 10G程序能够以与可提取的淀粉产量相同的顺序排列的羟基次序列。然而，对淀粉/麸质分离的杂交作用更加明显。用最低可提取淀粉回收杂种的含量较少的含淀粉。 10G程序将有利于测量早期几代玉米杂种的铣削效率，其中有限的样品可获得有限的样品，例如当表达有价值的重组蛋白时。

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