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Isolation of potato (Solanum tuberosum) dietary fibers, and analysis of their physical properties and sugar composition

机译:土豆(Solanum Tuberosum)膳食纤维的分离,并分析其物理性质和糖组合物

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Potato flesh and skin are rich sources of dietary fibers (DF). The soluble and insoluble DF of potato tubers were isolated from two potato varieties, Rangers Russet (RA) and Russet Burbank (RB). Hand peeled potato flesh was wet-fractionated into watersoluble and insoluble fractions. The insoluble fraction was wet-sieved through a screen of 105 micron openings to isolate insoluble dietary fibers (IDF) of 26~28% purity. The purity of IDF was further increased to ~85% by the removal of the remaining starch using heat stable alpha-amylase. Soluble dietary fiber (SDF) was isolated from the water soluble fraction through acid precipitation at pH 5 and removal of protein, and subsequent precipitation of fiber using 80% ethanol. Purity of SDF was increasedfrom 32~33% in the soluble fraction to 82~84%. The recovery of potato DF was 81.1~86.3% in IDF and 47.9~54.6% in SDF. Potato skins were collected by steaming the tubers and carefully hand-peeling. IDF of the skin fraction was purified by heat stable alpha-amylase to a purity of 89% with 67.3~76.2% recovery. Glucose was identified as a common sugar in all potato DF and in combination with arabinose, xylose, mannose, and fructose in IDF. Cellobiose, mannose, and galactose made up significant portions ofSDF. IDF of potato tubers exhibited significantly higher water holding capacity (mean 3.8ml/g) and oil binding capacity (7.7ml/g) in comparison to SDF and skin IDF. Swelling capacity of skin IDF (mean 4.82ml/g) was significantly higher than tuber IDF (mean 3.78ml/g).
机译:薯肉皮肤和皮肤是丰富的膳食纤维(DF)的来源。从两种土豆品种分离的马铃薯块茎的可溶性和不溶性DF,rangers russtrer(ra)和russtr urbank(Rb)。将手脱皮的马铃薯肉体湿法分离成水溶液和不溶性级分。通过105微米开口的筛网湿筛分不溶性级分,以分离不溶性膳食纤维(IDF)为26〜28%的纯度。通过使用热稳定的α-淀粉酶除去剩余的淀粉,IDF的纯度进一步增加到〜85%。通过酸沉淀在pH5下与水溶性馏分分离可溶性膳食纤维(SDF),除去蛋白质,并使用80%乙醇除去纤维的沉淀。 SDF的纯度在可溶性分数中增加32〜33%至82〜84%。薯DF的回收率在IDF中为81.1〜86.3%,SDF中的47.9〜54.6%。通过蒸块块和仔细手动剥离来收集马铃薯皮。通过热稳定的α-淀粉酶纯化皮肤级分的IDF,纯度为89%,回收率为67.3〜76.2%。葡萄糖被鉴定为所有马铃薯DF中的常用糖,并与阿拉伯语,木糖,甘露糖和IDF中的果糖组合。 Cellobiose,Mannose和半乳糖组成了大量的OFSDF。与SDF和皮肤IDF相比,马铃薯块茎的IDF具有显着更高的水持续容量(平均3.8mL / g)和油结合能力(7.7ml / g)。皮肤IDF(平均4.82ml / g)的溶胀容量明显高于块茎IDF(平均3.78ml / g)。

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