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首页> 外文会议>Shanghai International Conference on Laser Medicine and Surgery >Model for Monitoring the Process of Photodynamic Therapy in Patients
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Model for Monitoring the Process of Photodynamic Therapy in Patients

机译：监测患者光动力治疗过程的模型

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The photodynamic therapy (PDT) on tumors is quite effective and widely applied but usually carried out without an immediate evaluation of results. We measured the tumor fluorescence in mice with a fiber probe connected to a linear array spectral analyzer (PMA-11, Hamamatsu Photonics). The spectrum showed a transient change in fluorescence color from red to green during Photofrin~®-mediated PDT. In order to examine the source of green fluorescence, the mitochondria were accessed under a Nipkow disk-scanning confocal microscope in the HeLa cell in culture after labeling them with a red fluorescent protein (DsRedl-mito) and staining the cell with Photofrin~® (Axcan Scandipharm). Changes in fluorescence color from red to green were observed in the area of mitochondria upon their swelling during irradiation. This finding in vitro provided clear evidence that the change in fluorescence color from red to green observed in vivo was due to the mitochondrial destruction associated with the cell-death by PDT. This technique of spectral monitoring in tumor may be useful for detection of the cell-death signal during PDT in patients.
机译：肿瘤上的光动力疗法（PDT）非常有效和广泛应用，但通常在没有立即评估结果的情况下进行。我们用连接到线性阵列光谱分析仪（PMA-11，Hamamatsu光子）的纤维探针测量小鼠中的肿瘤荧光。在PhotoFrin〜®介导的PDT期间，光谱显示荧光颜色从红色到绿色的瞬态变化。为了检查绿色荧光的来源，在用红色荧光蛋白（DSREDL-MITO）标记培养后，在培养的Hela细胞中，在培养的Nipkow盘扫描共聚焦显微镜下进入线粒体。用PhotoFrin〜®染色电池（ Axcan Scandipharm）。在线粒体在辐射期间肿胀时观察到红色到绿色的荧光颜色的变化。这种发现体外提供了明确的证据表明，在体内观察到红色到绿色的荧光的变化是由于PDT与细胞死亡相关的线粒体破坏。这种肿瘤中的光谱监测技术可用于检测患者PDT期间的细胞死亡信号。

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