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Some new approaches to the detection of programmed cell death

机译：检测编程细胞死亡的一些新方法

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Apoptosis, or programmed cell death, is a form of cell death occurring during normal physiological processes and is used by the multicellular organism for elimination of "old" and impaired cells. Apoptosis is characterized by specific morphological changes such as plasma membrane blebbing, nucleus condensation, and cell wrinkling with further destruction into apoptotic bodies. Apoptosis detection is in focus of instrumental methods used in modern biomedical sciences. The available methods for such purpose are either very expensive, or require time-consuming operations. Their specificity and sensitivity are frequently not sufficient for biomedical diagnostics. We propose to use light scattering analysis for evaluation of apoptosis in cell population, especially for the detection of physical changes of cells, such as cell condensation and degradation into apoptotic bodies. The method proved to be very effective, providing quantitative estimation and high precision, simplicity and low costs of analysis (UA Patent No.64090). Another approach for the detection of apoptosis is based on the recently discovered fact (Bilyy, Stoika 2003; Bilyy et al, 2004; 2005) that apoptotic cells are characterized by increased expression levels of specific glycoproteins in the plasma membrane, which were proved to be selective and specific markers of apoptotic cells. Specific carbohydrate-binding proteins - lectins - were used for identification of mentioned glycoproteins; fluorescent conjugates of lectins were proved to be another novel tool for apoptosis identification using approaches of biophotonics.
机译：细胞凋亡或编程的细胞死亡是一种在正常生理过程中发生的细胞死亡形式，并且由多细胞生物体用于消除“旧”和受损的细胞。细胞凋亡的特征在于特定的形态学变化，例如血浆膜膨胀，核凝结和细胞皱纹，进一步破坏凋亡体。凋亡检测是在现代生物医学科学中使用的乐器方法的焦点。用于此类目的的可用方法是非常昂贵的或需要耗时的操作。它们的特异性和敏感性通常不足以用于生物医学诊断。我们建议使用光散射分析来评估细胞群中凋亡，特别是为了检测细胞的物理变化，例如细胞缩合和降解到凋亡体中。该方法证明是非常有效的，提供定量估计和高精度，简单性和分析成本低（UA专利No.64090）。另一种检测细胞凋亡的方法是基于最近发现的事实（Bilyy，Stoika 2003; Bilyy等，2004; 2005），凋亡细胞的特征在于质膜中的特定糖蛋白的表达水平增加，证明是凋亡细胞的选择性和特异性标记。特异性碳水化合物结合蛋白 - 凝集素 - 用于鉴定糖蛋白;证明章参荧光缀合物被证明是使用生物光源方法的方法是凋亡鉴定的另一个新工具。

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