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Monitoring of UVA-induced apoptosis in MCF-7 cells by light-scattering measurements

机译：通过光散射测量监测MCF-7细胞中的UVA诱导的细胞凋亡

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We report the use of light scattering to monitor the morphological changes in mammalian breast cancer cells (MCF-7) undergoing UVA irradiation induced apoptosis. The values for the scattering anisotropy parameter (g) and reduced scattering coefficient [μ_s/ = μ_s (1-g), where μ_s is the scattering coefficient] of the control cell suspensions and cell suspensions irradiated to varying dosage of UVA (80 - 160 kJ / m~2) were estimated for the wavelength range 450nm - 750 nm using integrating sphere measurements of diffuse reflectance and transmittance. These estimates were used to determine the Mie equivalent scatterer radius (r Mie) and thus quantify the morphological alterations of the cells. The value for r Mie was observed to decrease monotonically with increase in the UVA dose to up to a dose of 140 KJ/m2 and showed an increasing behavior beyond this dose. Since it is known that while apoptosis leads to shrinkage in volume of the cells and fragmentation of nuclei, necrosis of the cells is associated with a swelling of cells, the results suggest that at doses below 1 40 kJ/m2 cells undergo apoptosis and beyond this dose necrosis takes place. We also used the light scattering approach to monitor effect of He-Ne pre-irradiation on UVA induced damage in cells. Results were consistent with previous reports that suggest that He-Ne pre-irradiation can induce protection against the UVA damage to the cells.
机译：我们举报使用光散射来监测哺乳动物乳腺癌细胞（MCF-7）的形态变化，接受UVA辐照诱导的细胞凋亡。散射各向异性参数（g）的值和降低的散射系数[μ_s/ =μ_（1-g），其中μ_s是辐射到UV​​A的不同剂量的控制单元悬浮液和细胞悬浮液的散射系数（80 - 160使用漫反射率和透射率的集成球体测量估计波长范围450nm-750nm的KJ / M〜2）。这些估计用于确定MIE等效散射体半径（R mie），从而量化细胞的形态改变。观察到r mie的值随着UVA剂量的增加而单调，达到140kJ / m 2的剂量，并显示出超出该剂量的增加的行为。由于已知凋亡导致细胞体积的收缩和核的破碎，细胞坏死与细胞的肿瘤相关，结果表明，在低于1 40kJ / m2细胞的剂量下，经历细胞凋亡和超出此剂量坏死发生。我们还使用了光散射方法来监测He-ke预辐射对UVA在细胞中损伤的影响。结果与先前的报告一致，表明HE-NE预防可以诱导对细胞损伤的保护。

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