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Laminin Immobilized on Titanium Oxide Films for Enhanced Human Umbilical Vein Endothelial Cell Adhesion and Growth

机译:固定在氧化钛膜上固定的层内蛋白,以增强人脐静脉内皮细胞粘附和生长

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In prior work we have shown that titanium oxide (Ti-O) thin films have good blood compatibility. However, as well as being hemocompatible, biomaterials used in contact with blood should be cell compatible also. In the work described here, Ti-O films were synthesized using unbalanced magnetron sputtering (UBMS) and were modified by immobilizing laminin on the film surface for improving human umbilical vein endothelial cell (HUVEC) adhesion and growth. Scanning electron microscopy (SEM), Fourier Transform Infrared spectroscopy (FTIR) and contact-angle measurements were used to investigate the surface characteristics of the Ti-O films and the modified Ti-O films. The results suggest that Laminin can be biochemically immobilized on the Ti-O film surface. The modified layer of Laminin can improve the hydrophilicity and wettability of Ti-O films. In vitro HUVEC investigations reveal that Laminin immobilized on the film surface greatly enhances cell adhesion and growth on Ti-O films.
机译:在现有的工作中,我们已经表明氧化钛(Ti-O)薄膜具有良好的血液相容性。然而,除了血液中相容,使用与血液接触的生物材料也应该是细胞相容。在这里描述的工作中,使用不平衡的磁控溅射(UBMS)合成Ti-O膜,并通过固定膜表面上的层粘连蛋白来改变以改善人脐静脉内皮细胞(HUVEC)粘附和生长。扫描电子显微镜(SEM),傅里叶变换红外光谱(FTIR)和接触角测量用于研究Ti-O膜的表面特性和改性的Ti-O膜。结果表明,层粘连蛋白可以在Ti-O膜表面上生物化学加固。改性层状层可以改善Ti-O膜的亲水性和润湿性。体外HUVEC研究表明,固定在薄膜表面上的层粘蛋白大大提高了Ti-O膜的细胞粘附和生长。

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