• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>American Institute of Chemical Engineers Annual Meeting >Enhanced Osteoblast Adhesion, Proliferation and Differentiation on Nanocrystalline Diamond Coatings
【24h】

Enhanced Osteoblast Adhesion, Proliferation and Differentiation on Nanocrystalline Diamond Coatings

机译：增强纳米晶金刚石涂层上的成骨细胞粘附，增殖和分化

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Osteoblast (OB, bone forming cell) functions on diamond are critical for considering the use of anti-abrasive diamond coatings on orthopedic implants. In this study, OB functions including adhesion (up to 4 hrs), proliferation (up to 5 days) and differentiation (up to 28 days) on various diamond coatings were investigated. Two kinds of diamond coatings (nano- and submicron-crystalline diamond) were fabricated through microwave plasma enhanced chemical vapor deposition and characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM). Surface roughness and hydrophobicity of the diamond coatings increased dramatically as grain size grew. These coatings were tested for OB adhesion and proliferation by counting adherent cells after incubation of 4hrs up to 5 days. OB differentiation on diamond coatings after incubation from 1 to 3 weeks was investigated by measuring total intracellular protein synthesis, alkaline phosphatase activity, and calcium deposition. Results demonstrated enhanced OB functions on nanocrystalline diamond coatings compared to submicron grain size coatings. The long-term (up to 3 weeks) functions of OB on nanocrystalline diamond coatings were promoted compared to submicron diamond, polished silicon and glass coverslips. In summary, these results provided insights into the application of diamond coatings in orthopedics, which can potentially improve wear problems of current implants and prolong their lifetime.
机译：钻石上的Osteoooblast（OB，骨形成细胞）功能对于考虑在整形外科植入物上使用抗磨金金刚石涂层至关重要。在本研究中，研究了各种金刚石涂层上的抗粘连（最多4小时），增殖（最多5天）和分化（最多28天）的OB功能。通过微波等离子体增强化学气相沉积制造两种金刚石涂层（纳米和亚微晶金刚石），并通过扫描电子显微镜（SEM）和原子力显微镜（AFM）。随着晶粒尺寸的增长，金刚石涂层的表面粗糙度和疏水性显着增加。通过在温育至5天后测定粘附细胞，通过计数粘附细胞测试这些涂层，通过计数粘附细胞进行抗粘附性和增殖。通过测量总细胞内蛋白质合成，碱性磷酸酶活性和钙沉积，研究了在孵育后1至3周的金刚石涂层的差异化。结果表明，与亚微米粒度涂层相比，纳米晶金刚石涂层上的增强型OB功能。与亚微米金刚石，抛光硅和玻璃盖玻璃相比，促进了OB上的OB上的长期（最长3周）的函数。总之，这些结果提供了在骨科上应用金刚石涂层的见解，这可能会改善当前植入物的磨损问题并延长寿命。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号