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DNA Amplicons using Arbitrary Primers Distinguish Polymorphic Loci Among Mangrove Thraustochytrid Genomes

机译：DNA扩增子使用任意引物区分红树林中的多晶型位点

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Thraustochytrids - lightly-pigmented estuarine and marine microheterotrophs, taxonomically aligned with heterokont algae - are of great biotechnological interest because they produce substantial amounts of polyunsaturated fatty acids (PUFAs) especially docosahexaenoic acid (DHA:6n3). In this study, twenty-seven strains, isolated from twenty mangrove areas in the Philippine archipelago, were mass-produced in axenic flask cultures using high-glucose medium with continuous agitation. Polymorphic loci of thraustochytrid genomes, determined using four arbitrary primers (OPC02, OPC05, OPC07 and OPC08) in PCR analysis, were randomly amplified as molecular markers for genetic fingerprinting. Electrophoretic banding patterns of DNA amplicons, recognized based on nucleic acid size, were scored on data matrix and analyzed using Jaccard''s Coefficient and single-linkage hierarchical clustering to characterize degree of genetic relatedness among thraustochytrids. Conclusively, nearest-neighbor dendrogram of randomly amplified polymorphic DNAs (RAPDs) markers classify the strains into two monophenetic clades, representing the two major genera: Schizochytrium and Thraustochytrium.

机译：ThaustoChytrids - 轻质色素的河口和海洋微观疗法，与Heryozonong藻类分类 - 是巨大的生物技术的利益，因为它们产生大量的多不饱和脂肪酸（PUFA）特别是二十二碳六烯酸（DHA：6N3）。在本研究中，从菲律宾群岛中分离出207个菌株，在菲律宾群岛中的二十红树园中，在轴酮瓶中使用高葡萄糖培养基，具有连续搅拌。在PCR分析中使用四种任意引物（OPCO 2，OPCO 5，OPCO 7和OPCO 7）测定的Thraustochytrid基因组的多态基因座被随机扩增为遗传指纹识别的分子标记。基于核酸尺寸识别的DNA扩增子的电泳条带进行识别，并使用Jaccard'系数和单键分层聚类分析，以表征Thraustochytrids之间的遗传相关程度。结论，随机扩增多态DNA（RAPDS）标记的最近邻邻树木（RAPDS）标志物将菌株分为两种单体精液，代表两种主要属：Schizochytrium和Thraustochytrium。

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《Oceans - Europe》|2007年||共7页
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作者
Oclarit Jose M.; Hepowit Nathaniel L.;
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中图分类 P75-53;
关键词
RAPD fingerprinting; arbitrary primers; mangroves; polymorphic loci; thraustochytrids;

机译：Rapd指纹识别;任意引物;红树林;多晶型位点;Thraustochytrids;

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专利

1. DNA sequence divergence among derivatives of Escherichia coli K-12 detected by arbitrary primer PCR (random amplified polymorphic DNA) fingerprinting. [J] . I Brikun, K Suziedelis, D E Berg Journal of bacteriology . 1994,第6期

机译：通过任意引物PCR（随机扩增多态性DNA）指纹图谱检测到的大肠杆菌K-12衍生物之间的DNA序列差异。
2. DNA sequence divergence among derivatives of Escherichia coli K-12 detected by arbitrary primer PCR (random amplified polymorphic DNA) fingerprinting. [J] . I Brikun, K Suziedelis, D E Berg Journal of bacteriology . 1994,第6期

机译：通过任意引物PCR（随机扩增多态性DNA）指纹图谱检测到的大肠杆菌K-12衍生物之间的DNA序列差异。
3. Identification of an optimal set of random amplified polymorphic DNA primers to distinguish among ten strawberry cultivars in Korea and Japan. [J] . Je ByoungIl, Kim ChulMin, Piao HaiLong, Horticulture,Environment,and Biotechnology . 2007,第2期

机译：确定一组最佳的随机扩增多态性DNA引物，以区分韩国和日本的十个草莓品种。
4. DNA Amplicons using Arbitrary Primers Distinguish Polymorphic Loci Among Mangrove Thraustochytrid Genomes [C] . Oclarit, Jose M., Hepowit, . 2007

机译：DNA扩增子使用任意引物区分红树林破囊壶菌基因组中的多态性位点。
5. Construction of genetic linkage maps and locations of disease resistant and plant architectural loci in common beans using random amplified polymorphic DNA (RAPD) markers. [D] . Jung, Geunhwa. 1995

机译：使用随机扩增的多态性DNA（RAPD）标记构建普通豆中的遗传连锁图以及抗病和植物结构位点的位置。
6. DNA sequence divergence among derivatives of Escherichia coli K-12 detected by arbitrary primer PCR (random amplified polymorphic DNA) fingerprinting. [O] . I Brikun, K Suziedelis, D E Berg 1994

机译：通过任意引物PCR（随机扩增多态性DNA）指纹图谱检测到的大肠杆菌K-12衍生物之间的DNA序列差异。
7. DNA sequence divergence among derivatives of Escherichia coli K-12 detected by arbitrary primer PCR (random amplified polymorphic DNA) fingerprinting. [O] . Brikun, I, Suziedelis, K, Berg, D E 1994

机译：通过任意引物PCR（随机扩增多态性DNA）指纹图谱检测到的大肠杆菌K-12衍生物之间的DNA序列差异。
8. Random Amplified Polymorphic DNA (RAPD) Markers Readily Distinguish CrypticMosquito Species (Diptera: Culicidae: Anopheles) [R] . Wilkerson, R. C., Parsons, T. J., Albright, D. G., 1993

机译：随机扩增多态性DNa（RapD）标记易于区分隐蔽性蚜虫物种（双翅目：蚊科：按蚊）

DNA Amplicons using Arbitrary Primers Distinguish Polymorphic Loci Among Mangrove Thraustochytrid Genomes

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