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首页> 外文会议>International Conference on Pseudomonas Syringae Pathovars and Related Pathogens >Studies on Plant Pathogenic Bacterium CausalAgent of Soybean Bacterial Spots (Pseudomonassyringae pv. glycinea (Coerper) Young et al.)
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Studies on Plant Pathogenic Bacterium CausalAgent of Soybean Bacterial Spots (Pseudomonassyringae pv. glycinea (Coerper) Young et al.)

机译：大豆细菌斑的植物病原菌血管胰腺癌研究（糖尿病糖尿病糖尿病糖尿病糖尿嘧啶（Coerper）Young等人。）

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Bacterial blight is a disease of soybean appearing regularly with highintensity in Serbia. Isolation of pathogen was done using beef extract (MPA) andthe medium enriched with sucrose (NSA) from infected soybean leaves of severalvarieties grown in Vojvodina province, during 2005. Only the representative isolates were chosen for analyses. Pathogenicity of theisolates including the reference strain (National Collection of Plant PathogenicBacteria, United Kingdom-NCPPB 3318) was determined by inoculating soybeanplants at cotyledon stage (cv. Balkan), by dipping and spraying with bacterial sus-pensions (conc. 10~8 CFU/ml). Pathogenicity of isolates was also confirmed ontobacco plants (HR positive reaction). Cultural, biochemical and physiological characteristics were tested according tomethods cited by Lelliot and Stead (1987), Klement et al. (1990), Schaad (1980).The colonies are chosen by fluorescence on KB agar, and by the LOPAT tests (levanproduction, oxidase reaction, potato rot, arginine dihydrolase production andtobacco hypersensitivity: +,–,–,–,+). The studied isolates of bacterium did nothydrolyze starch, gelatine and aesculin. They did not reduce nitrate but metabolizedglucose oxidatively and were catalase positive. All the isolates produced acid fromglucose, galactose, xylose, arabinose, manose, sucrose, rafinose and inositol. Bacterium identification was confirmed by a serological method (agglutinationtests by Express Kit – NEOGEN Europe Ltd., Scotland, UK), with appropriateantibodies of Pseudomonas syringae pv. glycinea. The studied isolates formedwhitish sediment in the case of positive reaction.
机译：细菌枯萎是塞尔维亚的高度高度出现的大豆疾病。使用牛肉提取物（MPA）和富含蔗糖（NSA）的培养基的分离从Vojvodina省生长的几八粒中富集的蔗糖（NSA）进行，选择了代表性分离物用于分析。通过在子叶阶段（CV.BALKAN）的豆荚植物，通过浸入细菌SUS-养老金（CONG.10〜8 CFU）通过接种和喷涂来确定包括参考菌株（国家植物致病菌的植物致病菌植物致病菌，联合王国-NCPPB 3318）（CONG.10〜8 / ml）。分离株的致病性也证实了一只乌布布（HR阳性反应）。根据Lelliot和Stead（1987），Klement等人所引用的修饰，测试了文化，生化和生理特征。 （1990），Schaad（1980）。菌落在Kb琼脂上选择荧光，并通过LoPat试验（LevanProduction，氧化酶反应，马铃薯腐，精氨酸二氢酶生产雄段超敏反应：+， - - ， - ，+）。学习的细菌分离株Notfrolyze淀粉，明胶和紫叶蛋白。它们没有减少硝酸盐，但氧化氧化葡萄糖并被过氧化酶阳性。所有分离物产生的酸，半乳糖，木糖，阿拉伯糖，蔗糖，蔗糖，罗法糖和肌醇。通过血清学方法（Calthutinalsts通过Express Kit-Neogene Europers Ltd.，UK）的血凝法来证实细菌鉴定，具有Pseudomonas inringae PV的申请。甘氨酸。研究的分离物在阳性反应的情况下环形沉积物。

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