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首页> 外文会议>International Conference on Pseudomonas Syringae Pathovars and Related Pathogens >Genomic Analysis of Pseudomonas syringaePathovars: Identification of Virulence Genesand Associated Regulatory Elements UsingPattern-Based Searches and Genome Comparison
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Genomic Analysis of Pseudomonas syringaePathovars: Identification of Virulence Genesand Associated Regulatory Elements UsingPattern-Based Searches and Genome Comparison

机译：假鼠霉素的基因组分析：基于模巴的搜索和基因组比较的毒力遗传学和相关调节元件的鉴定

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The availability of complete genome sequences for three P syringaepathovars (P s. pv. tomato DC3000, P s. pv. phaseolicola 1448A, and P s. pv.syringae B728a) provides researchers with unprecedented opportunities to explorefactors contributing to pathogenicity and virulence in these pathogens. One methodemployed for genome sequence analysis involves identification of genes andregulatory elements through association with conserved sequence motifs. Usingthis approach, ~50 and~30 genes for T3SS-dependent Hop/Avr effector proteinshave been identified in Pst DC3000 and Psp 1448A, respectively, on the basisof their proximity to conserved "hrp box" regulatory motifs and the presence ofHop-associated patterns at the N-terminus of the encoded proteins. These analyseshave also led to a comprehensive catalog of the hrpL regulons of all three pathovars.Similar pattern-based searches have led to comprehensive identification of otherregulatory elements in the Pst DC3000 genome, including binding sites for RpoD,RpoE, RpoN, RpoS, and Fur. Genome comparison represents a second powerfultool for identification of the genes and regulatory elements involved in diversestages of the plant—pathogen association. For example, genome comparisonof the three sequenced P syringae pathovars sheds light on genes contributingto their different pathogenic strategies. More broadly, genes distinguishing theplant-associated pseudomonads from pseudomonads occupying other niches canbe identified as described in the study comparing the Pst DC3000 genome withthat of the saprophyte P putida and the animal pathogen P. aeruginosa. Using thisapproach, 44 "LSRs" (lineage-specific regions) were identified in the Pst DC3000genome.
机译：三个P syringaepathovars的完整基因组序列的可用性（p s.pv。番茄DC3000，p s. pv。phaplingolicola 1448a和p s.pv.syringae b728a）为研究人员提供了前所未有的机会，从而利用这些致病性和毒力病原体。用于基因组序列分析的方法均可涉及通过与保守的序列基序相关联的基因和调节元件的鉴定。使用该方法，在PST DC3000和PSP 1448A中，在PST DC3000和PSP 1448A中鉴定了T3SS依赖跳/ AVR效应器蛋白蛋白的基因，其基础是它们的邻近的保守的“HRP盒”调节基序和相关图案的存在编码蛋白的N-末端。这些分析还导致了所有三个帕夫多瓦洛夫的HRPL稳态的全面目录。基于模式的搜索导致了PST DC3000基因组中其他调节元素的综合鉴定，包括RPOD，RPOE，RPON，RPO和皮草的结合位点。基因组比较代表了用于鉴定参与植物病原体结论的分类器的基因和调节元件的第二种Powerfulitool。例如，三个测序的P syringae Patovars的基因组比较揭示了促进其不同致病策略的基因。更广泛地，将相关联的假单胞菌的基因与占据其其他核桃的假单胞菌相同，如研究中的描述，比较PST DC3000基因组与Saprophyte P普韦达和动物病原体P. eruginosa的研究。使用TheApproach，在PST DC3000GANOME中识别44“LSRS”（谱系特定区域）。

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