• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>International Conference on Environmental, Industrial and Applied Microbiology >Identification of virulence genes in Fusarium oxysporum f. sp. lycopersici the causal agent of tomato wilt disease
【24h】

Identification of virulence genes in Fusarium oxysporum f. sp. lycopersici the causal agent of tomato wilt disease

机译：牡蛎中毒力基因的鉴定。 sp。 Lycopersici番茄枯萎病的因果剂

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Fusarium oxysporum f. sp. lycopersici is the causal agent of tomato wilt disease. The identification of SIX genes was performed using primers SIX1, SIX2, SIX3 and SHH1. PCR with the SIX1, SIX2, SIX3 and SSH1 primers set amplified a 647~726-bp fragment from three races of the F. oxysporum. Sequence analysis was performed on an ABI 3700 DNA Analyzer. The sequences obtained were compared with the Six genes sequences available from GenBank using a BLASTn search. The gene six1 be grouped in one clade where races 1 and 2 showed a genetic identity of 100% and a similarity of 98%, while race 3, the similarity was lower (85%). For six2, and six3 shh1 genes, the race 3 showed a very different behavior from races 1 and 2. Where identity (56%, 50%, 50%) and similarity (65%, 65%, 60%) were much lower than in races 1 and 2, respectively. Our findings have practical implications for the detection and identification of f.sp. lycopersici, these genes may be part of a larger, dispensable region of the genome that confers the ability to cause tomato wilt and has spread among clonal lines of F. oxysporum through horizontal gene transfer. Identification of genomic regions contributing to the distinction of races when combined with other markers and may help the development of molecular markers race-specific to be used in the characterization of isolates of F. oxysporum sp. lycopersici circulating in different counties tomato growers of the state of Pernambuco and Brazil.
机译：Fusarium oxysporum f。 sp。 Lycopersici是番茄枯萎病的因果因子。使用引物六，62,63和SHH1进行六种基因的鉴定。 PCR用Six1,62，Six3和SSH1引物集合扩增来自F. oxysporum的三场比赛的647〜726-bp片段。对ABI 3700 DNA分析仪进行序列分析。将获得的序列与从Genbank获得的六个基因序列进行比较，使用BLASTN搜索。基因六1在一个曲线中分组，其中步伐1和2显示遗传标识为100％和98％的相似性，而参数3，相似性较低（85％）。对于S162和S163 SHH1基因，比赛3显示了比赛1和2的非常不同的行为。其中身份（56％，50％，50％）和相似性（65％，65％，60％）远低于在比赛1和2中。我们的研究结果对F.SP的检测和识别有实际意义。 Lycopersici，这些基因可能是基因组的较大，可分配区域的一部分，其赋予引起番茄枯萎的能力，并通过水平基因转移在F. oxysporum的克隆系中传播。鉴定与其他标记相结合的种族区别造成种族的基因组区域，并且可以帮助开发用于特异性的分子标记种血液标记物的特异性用于氧血纯度Sp的分离株的表征。 Lycopersici在不同县的番茄种植者中流通，佩尔南多和巴西的国家。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号