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首页> 外文会议>International Conference on Environmental, Industrial and Applied Microbiology >Growing kinetics and antimicrobial activity of Streptomyces tubercidicus crude extracts
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Growing kinetics and antimicrobial activity of Streptomyces tubercidicus crude extracts

机译：生长动力学和抗菌素的乳酸霉素粗提取物的动力学和抗菌活性

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Streptomyces spp. is studied in function of its antibiotic production with industrial and pharmaceutical applications. This work aimed to evaluate the growing kinetics of the endophytic S. tubercidicus isolated from Solanum lycocarpum St. Hill, a typical Brazilian tropical savannah tree, and compares its bioactivity against Staphylococcus aureus ATCC 29213 and Escherichia coli ATCC 25922. S. tubercidicus was cultivated in ISP2 broth and its growing kinetics was evaluated during 21 days. The major quantification measured was 7.88 log CFU.ml~(-1) seen at the second day of incubation. The bioactive substances production was seen at the end of the exponential phase and the beginning of the stationary phase of growth (day two). The fresh crude extract showed a maximum antimicrobial potential of 200 AU.mL~(-1) against Escherichia coli and Staphylococcus aureus. The crude extract was active during 21 days, without modification of its antimicrobial potential and revealed three compounds using thin layer chromatography under UV illumination.
机译：Streptomyces spp。在其具有工业和制药应用的抗生素生产的功能中研究。这项工作旨在评估从典型的巴西热带大草原树中分离的生成的Cubercidicus的生长动力学，典型的巴西热带大草原树，并将其对金黄色葡萄球菌ATCC 29213和大肠杆菌ATCC 25922的生物活性进行了比较。培养了Tubercidicus。 ISP2肉汤及其生长动力学在21天内进行了评估。测量的主要量化为7.88 Log Cfu.ml〜（-1）在孵化的第二天看到。在指数阶段结束时看到生物活性物质产生和生长的固定阶段（第二天）的开始。新鲜粗提取物显示出200AU.ML〜（-1）的最大抗菌潜力，其对大肠杆菌和金黄色葡萄球菌。在21天内，粗提取物活跃，无需改变其抗微生物电位，并在紫外线照射下使用薄层色谱法揭示了三种化合物。

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