Salmonella enterica Typhimurium: establishment of cultivation condition on shake flask and flagellin isolation strategy by using tangencial ultrafiltration

机译：Salmonella肠道毒蕈类动物：使用切向超滤，在摇瓶和鞭毛蛋白隔离策略中建立培养条件

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Flagellin is a main protein present in the bacterial flagellum and its molecular mass is around 51.5 kDa. Its role as an adjuvant vaccine has been intensively studied and currently there is evidence to be an immunomodulador in situations of autoimmune diseases, co-administered with chemotherapy or as radioprotetor. This study focus on to establish the native flagellin production by Salmonella Typhimurium in shake flask and flagellin purification strategy by using tangencial ultrafiltration. Results showed that flagellin can be released to the supernatant depend on the agitation speed, and tangencial ultrafiltration can be used as tool to purify this protein.

机译：鞭毛蛋白是细菌鞭毛中存在的主要蛋白质，其分子量为51.5kDa。它作为佐剂疫苗的作用被密集地研究，目前有证据表明是在自身免疫性疾病的情况下是一种免疫调节因子，与化疗或辐射丙丙丙呤共同施用。本研究重点是通过使用切割超滤来建立由Salmonella Typhimurium在摇瓶和鞭毛蛋白净化策略中的鞭毛生产。结果表明，鞭毛蛋白可以释放到上清液上取决于搅拌速度，并且切割超滤可以用作纯化该蛋白质的工具。

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《International Conference on Environmental, Industrial and Applied Microbiology》|2009年||共4页
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B.H. Oliveira; M.R. Silva; C.J.M. Braga; L.M. Massis; L.C.S. Ferreira; M.E. Sbrogio-Almeida; M. Takagi;
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中图分类 Q939.9-532;
关键词
Salmonella; flagellin; homogeneizer; tangencial ultrafiltrati;

机译：沙门氏菌;鞭毛;均质器;切向超滤;

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专利

1. Isolation of Salmonella enterica subsp. enterica (O:4,5:i) and Salmonella enterica subsp. Typhimurium from free-living domestic pigeons (Columba livia) [J] . Arquivo Brasileiro de Medicina Veterinaria e Zootecnia . 2014,第5期

机译：肠炎沙门氏菌亚种的分离。 enterica（O：4,5：i）和沙门氏菌肠亚种。自由生活家鸽的鼠伤寒（Columba livia）
2. Outer membrane vesicles from flagellin-deficient Salmonella enterica serovar Typhimurium induce cross-reactive immunity and provide cross-protection against heterologous Salmonella challenge [J] . Qiong Liu, Qing Liu, Jie Yi, Scientific reports. . 2016,第1期

机译：来自鞭毛蛋白缺乏沙门氏菌的外膜囊泡肠道肠毒鼠诱导杂交反应性免疫力，并提供对异源沙门氏菌攻击的交叉保护
3. Rhizosphere effect on survival of Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium in manure-amended soil during cabbage (Brassica oleracea) cultivation under tropical field conditions in Sub-Saharan Africa. [J] . Ongeng D, Muyanja C, Ryckeboer J, International Journal of Food Microbiology . 2011,第2期

机译：热带环境下白菜（ Brassica oleracea ）栽培过程中根际对粪肥改良土壤中大肠埃希菌O157：H7和肠沙门氏菌鼠伤寒存活的影响撒哈拉以南非洲的油田条件。
4. Salmonella enterica Typhimurium: establishment of cultivation condition on shake flask and flagellin isolation strategy by using tangencial ultrafiltration [C] . B.H. Oliveira, M.R. Silva, C.J.M. Braga, International Conference on Environmental, Industrial and Applied Microbiology . 2009

机译：Salmonella肠道毒蕈类动物：使用切向超滤，在摇瓶和鞭毛蛋白隔离策略中建立培养条件
5. Structure-function studies of self-assembling flagellin proteins from Salmonella typhimurium and Aquifex pyrophilus. [D] . Malapaka, Venkata Raghu Ram. 2007

机译：鼠伤寒沙门氏菌和嗜热Aquifex嗜热菌自组装鞭毛蛋白的结构功能研究。
6. Outer membrane vesicles from flagellin-deficient Salmonella enterica serovar Typhimurium induce cross-reactive immunity and provide cross-protection against heterologous Salmonella challenge [O] . Qiong Liu, Qing Liu, Jie Yi, -1

机译：鞭毛蛋白缺陷型肠炎沙门氏菌鼠伤寒沙门氏菌的外膜囊泡可诱导交叉反应性免疫并提供针对异源沙门氏菌攻击的交叉保护作用
7. Isolation of Salmonella enterica subsp. enterica (O:4,5:i) and Salmonella enterica subsp. Typhimurium from free-living domestic pigeons (Columba livia) [O] . R.C. Rocha-e-Silva, W.M. Cardoso, R.S.C. Teixeira, 2014

机译：肠沙门氏菌亚种的分离enterica（O：4,5：i）和肠沙门氏菌亚种。来自自由生活的家养鸽子的Typhimurium（Columba livia）

Salmonella enterica Typhimurium: establishment of cultivation condition on shake flask and flagellin isolation strategy by using tangencial ultrafiltration

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