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Transcriptional regulation of ADE2 and PUT2 genes in Kluyveromyces lactis

机译:ADE2和PUT2基因在Kluyveromyces Lactis中的转录调节

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Two genomic fragments containing the KlADE2 and the KlPUT2 genes were isolated from Kluyveromyces lactis genomic library by functional complementation of the corresponding ade2 and put2 mutations in Saccharomyces cerevisiae. The deduced KlAde2p and KlPut2p amino acid sequences displayed strong similarities to their counterparts in Saccharomyces cerevisiae (75% and 72% identity respectively) with highly conserved domains. Thus, we report the isolation and characterization of ADE2 and PUT2 genes from Kluyveromyces lactis, including their protein structure, flanking sequence regions, and transcriptional gene regulation by different nutrients in the medium.
机译:通过相应的ADE2和Saccharomyces Cerevisiae中的相应ADE2和PUT2突变的功能互补,从Kluyveromyces乳酸基因组文库中分离出含有Klade2和Klput2基因的两个基因组片段。推导的klade2p和klput2p氨基酸序列与具有高度保守结构域的酿酒酵母(分别为75%和72%的身份)的对应物具有强烈的相似性。因此,我们报告了来自Kluyveromyces乳房的ADE2和PUT2基因的分离和表征,包括其蛋白质结构,侧翼序列区域和培养基中不同营养物的转录基因调节。

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