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首页> 外文会议>International Conference on Environmental, Industrial and Applied Microbiology >Standardisation and optimisation of the Alkaline-Tolerance Response (AlTR) in Listeria monocytogenes 10403S
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Standardisation and optimisation of the Alkaline-Tolerance Response (AlTR) in Listeria monocytogenes 10403S

机译：李斯特菌单核细胞增生碱耐药响应（ALTR）的标准化和优化10403s

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Listeria spp. can adapt to, and survive in, higher alkaline pH values than many other bacteria. This adaptation allows this important food-borne pathogen to resist subsequent (otherwise lethal) extreme alkaline stress, i.e. exposure to pH 12.0. Although such "stress hardening" may have significant public health implications by increasing the persistence of L. monocytogenes in food processing environments cleaned with alkali detergents, relatively little is known about the mechanisms and processes involved in this Alkaline-Tolerance Response (AlTR). This study examined the impact of growth phase, (alkali) pH conditions and exposure period in the extent of AlTR induction in L. monocytogenes. The extent of the AlTR (defined in terms of survival rates of adapted cells to subsequent pH 12.0 challenge) was observed to be growth-phase dependent, with significantly more cells surviving in exponential cell suspensions than in stationary cell suspensions. AlTR was induced between pH 8.0 to pH 10.0 with maximal induction (maximum cell survival) at pH 9.5. AlTR was rapidly expressed, with significantly more cells surviving in adapted suspensions (pH 9.5) for periods as short as 15 min, than in control (pH 7.4) suspensions. L. monocytogenes displayed different alkali responses on exposure to different types of alkali. These results confirm the hypothesis that L. monocytogenes responds to alkali pH through a complex network of responses that result to a phenotypic gain of resistance depending on many biological variables. In view of the fact that alkaline conditions are commonly encountered processing/preservation and clinical environments, these responses have profound implications to public health.
机译：listeria spp。可以适应和存活，比许多其他细菌更高的碱性pH值。这种适应允许这种重要的食物传播的病原体来抵抗随后的（否则致命的）极端碱性应力，即暴露于pH12.0。虽然这种“应力硬化”可能具有通过增加用碱洗涤剂清洁的食品加工环境中的L.单核细胞增生的持续性具有重要的公众健康影响。关于该碱性耐受响应（ALTR）所涉及的机制和方法是相对较少的。本研究检测了在L.单核细胞增生中的Altr诱导方面的生长阶段，（碱）pH条件和暴露期的影响。观察到Altr的范围（在适应细胞的适应细胞的存活率方面定义）是依赖于生长相的生长相，在指数细胞悬浮液中存活的细胞显着多于固定细胞悬浮液。在pH8.0至pH10.0之间诱导ALTR，具有最大诱导（最大细胞存活）pH9.5。 ALTR迅速表达，显著更多的细胞在适于悬浮液（pH 9.5）中对存活期短至15分钟，比对照（pH 7.4）中的悬浮液。 L.单核细胞元对暴露于不同类型的碱进行不同的碱响应。这些结果证实了L.单核细胞增生通过复杂的响应网络通过复杂的响应网络响应碱性pH值，这取决于许多生物变量的抗性的表型增益。鉴于碱性条件通常遇到加工/保护和临床环境，这些反应对公共卫生产生了深远的影响。

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