• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>International Conference on Environmental, Industrial and Applied Microbiology >Optimization of Neisseria lactamica's outer membrane vesicles production in batch cultivation process
【24h】

Optimization of Neisseria lactamica's outer membrane vesicles production in batch cultivation process

机译：分批栽培过程中Neisseria Lactamica外膜囊泡的优化

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Outer membrane vesicles (OMV) are released by evaginations of the outer membrane of Gram negative bacteria and have gained attention with respect to their biological functions, role in pathogenesis and use in new vaccines strategies and combinations. OMV from Neisseria lactamica induce antibodies that cross-react with Neisseria meningitidis and can have a potential mucosal adjuvant property. The objective of this work was to optimize OMV production from N. lactamica through the modification and improvement of its original culture medium, meningococcus defined medium (MCDA). Experiments were carried out in batch processes which had their concentration of lactate, amino acids and yeast extract (YE) modified according to each essay. Results indicated that the use of threefold of MCDA's concentration of lactate and double of its concentration of amino acids plus 2 g/l of YE seems to be the most favourable condition in both terms of cost and productivity of OMV. This proportion between carbon and nitrogen sources appears to be the most adequate for N. lactamica's metabolism.
机译：外膜囊泡（OMV）由革兰氏阴性菌外膜的evaginations释放，并获得了重视与发病机制对于它们的生物学功能，作用和使用新疫苗的策略和组合。来自Neisseria Lactamica的OMV诱导与脑膜炎脑炎尼霉菌交叉反应的抗体，并且可以具有潜在的粘膜佐剂性能。这项工作的目的是通过其原始培养基，脑膜炎球菌定义的培养基（MCDA）来优化N.乳酰酰酰胺的OMV生产。在分批过程中进行实验，其浓缩乳酸浓度，氨基酸和酵母提取物（YE）根据每个文章进行修饰。结果表明，使用三倍的MCDA浓度的乳酸浓度和两倍浓度的氨基酸加2 G / L似乎是OMV的成本和生产率的最有利条件。碳和氮源之间的这种比例似乎是N.Nactamica的新陈代谢最适合。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号