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首页> 外文会议>International symposium on environmental science and technology >Effect of Encapsulated and Free Isolated Lactobacillus, Lactobacillus Plantarum ATCC 8014 on Fermentation, Aerobic Stability of Corn Silage
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Effect of Encapsulated and Free Isolated Lactobacillus, Lactobacillus Plantarum ATCC 8014 on Fermentation, Aerobic Stability of Corn Silage

机译：包封和自由乳菌菌，乳杆菌植物杆菌ATCC 8014对发酵，有氧稳定性的玉米青贮饲料

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Ensiling is a preservation method for moist forage crops. It is based on lactic acid bacteria (LAB) converting water-soluble carbohydrates (WSC) into organic acids, mainly lactic acid, under anaerobic conditions. As a result, pH decreases and the moist forage is preserved from spoilage by microorganisms. Probiotics are "Living microorganisms ", which have health benefits for human and animals. The purpose of the present work was to study isolation of lactic acid bacteria from silage and the effects of isolated bacterium in combination with homofermentative LAB, on the fermentation, aerobic stability of cornsilage. In this study, the specie of Lactobacillus has been isolated from corn silage from cold region by anaerobic culture. The Biochemical tests were performed. Also, acid tolerance and growth in bile salt were measured. With due attention to low stability of probiotics in environment, these bacteria were encapsulated with alginate and chitosan. The effect of isolated Lactobacillus and Lactobacillus plantarum (free, encapsulated), on the fermentation, aerobic stability corn silage was studied. Silages with no additives served as control. Two jars from per treatment were sampled on days 0, 7, 15, 30 and 45. After 45 day of storage, silages were subjected to an aerobic stability test lasting 15 days, in which CO2 production, as well as chemical and microbiological parameters, were measured to determine the extent of aerobic deterioration. This study showed that the isolated bacterium is Lactobacillus spp. Also, at the end of the ensiling period (day 45), the isolated Lactobacillus+L. plantarum-inoculated silages had significantly higher stability than the control and encapsulaed bacteria-inoculated silages. The results showed that probiotics effectively could be used for increase of silage stability.
机译：禁止是滋生饲料作物的保存方法。它基于乳酸菌（实验室）将水溶性碳水化合物（WSC）转化为有机酸，主要是乳酸在厌氧条件下。结果，pH降低，潮湿的饲料被微生物腐败保存。益生菌是“生活微生物”，对人类和动物具有健康益处。本工作的目的是从青贮乳酸菌的研究分离和分离的细菌的结合同型发酵LAB的影响，对发酵，cornsilage有氧稳定性。在这项研究中，通过厌氧培养物从寒冷地区的玉米青贮血清中分离出乳杆菌。进行生化试验。此外，测量胆汁盐中的耐酸性和生长。由于适当注意环境中益生菌的低稳定性，这些细菌用藻酸盐和壳聚糖包封。研究了分离的乳杆菌和乳杆菌（自由，包封），对发酵，有氧稳定性玉米青贮饲料的影响。无添加剂的青贮饲料用作控制。每次治疗的两个罐子被取样在第0,7,15,30和45天。在45天的储存后，在持续15天内进行有氧稳定性测试，其中CO 2生产，以及化学和微生物参数，测量以确定有氧劣化程度。这项研究表明，孤立的细菌是乳酸杆菌SPP。此外，在禁止期间（第45天）结束时，分离的乳杆菌+ L。 Platarum接种的青贮血散比对照和包裹的细菌接种的混合物显着更高。结果表明，益生菌可有效地用于增加青贮淤稳定性。

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