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首页> 外文会议>International Conference on Manufacturing Science and Engineering >Studies on the preliminary characterization of a novel exopolysaccharide produced by Streptococcus thermophilus strain from Tibetan kefir grain
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Studies on the preliminary characterization of a novel exopolysaccharide produced by Streptococcus thermophilus strain from Tibetan kefir grain

机译：藏谷粒中链球菌菌株生产的新型外糖的初步特征研究

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The novel exopolysaccharide (EPS) was produced by a streptococcus thermophilus strain isolated from Tibetan kefir grain, and it was purified using DEAE cellulose 52 and DEAE Sepharose CL-6B column chromatography. Then it was eluted as a single symmetrical narrow peak on high-performance gel-permeation chromatography (HPGPC) and the average molecular mass was estimated to be 30,092Da. Infrared spectrum analysis indicated it had characteristic glycosidic structures. Gas chromatography showed that the EPS is composed of mannose and glucose in a molar ratio of 2.6:1 with trace galactose. The structural features of EPS were investigated by partial hydrolysis with acid, methylation analysis and NMR spectroscopy analysis. The results revealed that the main backbone chain of EPS was (2→3)-β-D-Manp, (3→4)-β-D-Manp, (2→3,6)-α-D-Glcp and trace amounts of galactose.
机译：新型外偶（EPS）是由来自藏科谷粒中分离的链球菌嗜热菌株产生的，并使用DEAE纤维素52和DEAE Sepharose CL-6B柱色谱法纯化。然后在高性能凝胶渗透色谱（HPGPC）上被洗脱作为单一对称窄峰，估计平均分子量估计为30,092DA。红外光谱分析表明它具有特征性糖苷结构。气相色谱显示，EPS由摩尔比的甘露糖和葡萄糖组成2.6：1的痕量半乳糖。通过用酸，甲基化分析和NMR光谱分析来研究EPS的结构特征。结果表明，EPS的主要骨干链是（2→3）-β-D-MANP，（3→4）-β-D-MANP，（2→3,6）-α-D-GLCP和痕迹半乳糖的量。

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