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Construction of Expression Vector with Fruit-Specific Promoter and Genetic Transformation of Strawberry

机译：表达载体的构建与果实特异性启动子和草莓遗传转化

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In order to transcription and expression an exogenous gene just in transgenic strawberry fruit, the gene encoding tomato fruit-specific E8 promoter was cloned. We constructed plant expression vector with E8 promoter replace the CaMV35S of pBI121 that used for transformation of strawberry. Recombination plasmid was identified by PCR, restriction enzymes digestion and sequencing analysis, and transfered into the agrobacterium EHA105 strain. At the same time, the agrobacterium EHA105, transformation and no transformation tissue of strawberry used as the experiment materials to study the expression of reporter gene gusA by the histochemical staining method. The results show that agrobacterium which contains gusA gene and strawberry organization of transformation can be dyed blue, others were not stain. So in testing the transgenic plants by agroinfiltration transient expression system can reduce or avoid false positive appearance.
机译：为了在转基因草莓果中转录和表达外源基因，克隆了编码番茄果实特异性E8启动子的基因。我们用E8启动子构建植物表达载体取代了用于转化草莓的PBI121的CAMV35。通过PCR，限制酶消化和测序分析鉴定重组质粒，并转移到Agrobacterium EHA105菌株中。同时，用作实验材料的草莓农杆菌，转化和没有转化组织，通过组织化学染色方法研究报告基因GUSA的表达。结果表明，含有Gusa基因和草莓转化组织的土壤杆菌可以染色蓝色，其他没有染色。因此，在通过农毒素过滤瞬态表达系统测试转基因植物时可以减少或避免假阳性外观。

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