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首页> 外文会议>International Conference on Manufacturing Science and Engineering >High-purity isolation of frans-resveratrol from Rhizma Polygoni Cuspidati by high-speed counter-current chromatography
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High-purity isolation of frans-resveratrol from Rhizma Polygoni Cuspidati by high-speed counter-current chromatography

机译：通过高速逆流色谱法从Rhizma Polygoni Cuspidati的高纯度分离弗拉斯 - 白藜芦醇

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摘要

In this paper, high-speed counter-current chromatography (HSCCC) was established and successfully applied for the separation and purification of trans-resveratrol from Rhizma Polygoni Cuspidati. After extracted by microwave assisted extraction (MAE), the diy extract was directly dissolved in HSCCC solvent and introduced to HSCCC separation system. Two-phase solvent system of HSCCC was optimized. trans-resveratrol was separated from extract of Rhizma Polygoni Cuspidati by high-speed counter-current chromatography in two-step using two different solvent systems composed of chloroform-methanol-water (4:3:2, v/v/v) and n-hexane-ethyl acetate-methanol-water (3:5:4:6, v/v/v/v). From 200 mg of crude extract, 2.5 mg of trans-resveratrol was obtained at over 99% purity by HPLC analyses, and its chemical structure was identified by ESI-MS, ~1HNMR.
机译：本文建立了高速逆流色谱（HSCCC），并成功地应用于Rhizma Polygoni Cuspidati的分离和纯化转霉醇。通过微波辅助萃取（MAE）提取后，DIY提取物直接溶解在HSCCC溶剂中并引入HSCCC分离系统。优化了HSCCC的两相溶剂体系。通过使用由氯仿 - 甲醇 - 水（4：3：2，v / v）和n的两种不同的溶剂系统，通过两步中的高速逆流色谱法分离Relizma Polygoni Cuspidati的提取物。 - 乙酯 - 乙酸乙酯 - 甲醇 - 水（3：5：4：6，v / v / v / v）。从200mg的粗提物中，通过HPLC分析得到超过99％的纯度获得2.5mg反式白藜芦醇，通过ESI-MS，〜1HNMR鉴定其化学结构。

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