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首页> 外文会议>Conference on Environmental Science and Information Application Technology >Effect of Juglone in Qinglongyi on cell cycle status and apoptosis in A-549 cells
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Effect of Juglone in Qinglongyi on cell cycle status and apoptosis in A-549 cells

机译：juglone在清隆义对A-549细胞细胞周期状态和细胞凋亡的影响

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To explore the inhibition of juglone in Qinglongyi on A-549 cells in vitro, MTT assay was used. Laser confocal scanning microscope was used to observe apoptotic morphology. Changes of cell cycle are studied by flow cytometry analysis. The result of MTT assay showed that juglone had a marked growth inhibition in A-549 cells and the IC50 is respectively 3.4×10~(-5) mol/L, 1.8×10~(-5)mol/L and 2.6×10~(-6)mol/L after treatment for 24, 48 and 72 h by juglone. Through Laser confocal scanning microscope, we can see that juglone can induce the apoptosis. Cell cycle changes are analyzed by flow cytometry with cells at Gi phase significantly less than those of control and cells at G_2 phase significantly more than those of control. It suggests that juglone could apoptosis of A-549 cells with the cell cycle arrest on G_2 phase in distinct dose-dependent manner.
机译：为了探讨在体外A-549个细胞上juglone对juglone抑制juglone，使用MTT测定。激光共聚焦扫描显微镜用于观察细胞凋亡的形态。通过流式细胞术分析研究细胞周期的变化。 MTT测定的结果表明，Juglone在A-549细胞中具有明显的生长抑制，IC50分别为3.4×10〜（-5）Mol / L，1.8×10〜（-5）Mol / L和2.6×10 〜（-6）MOL / L在Juglone处理24,48和72小时后。通过激光共焦扫描显微镜，我们可以看到Juglone可以诱导细胞凋亡。细胞周期通过流式细胞术进行分析，所述流式细胞术在GI期细胞显着小于G_2相的对照和细胞显着多于对照。它表明，Juglone可以以不同剂量依赖性方式对G_2相的细胞周期停滞凋亡的A-549细胞凋亡。

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