The extraction of polysaccharides from cordyceps militaris by ethanol refluxing method was studied and the effected of solvent, solid-liquid ratio and time on the yields of polysaccharides were investigated and optimized conditions were determined through L9(34) orthogonal test at the same time. The yield of validate experiment was 33.50% and the combination was 50% ethanol + 1:15(g/ml) solid-liquid ratio + 7 h extraction time.
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