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首页> 外文会议>Uppsala Conference School on Electron Capture and Transfer Dissociation >COMBINING FULLY AUTOMATED CHIP-BASED ELECTROSPRAY IONIZATION AND MULTISTAGE CID/ETD MASS SPECTROMETRY FOR HIGH-THROUGHPUT STRUCTURAL ANALYSIS OF GLYCOPEPTIDES
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COMBINING FULLY AUTOMATED CHIP-BASED ELECTROSPRAY IONIZATION AND MULTISTAGE CID/ETD MASS SPECTROMETRY FOR HIGH-THROUGHPUT STRUCTURAL ANALYSIS OF GLYCOPEPTIDES

机译：结合全自动芯片电喷雾电离和多级CID / ETD质谱法以进行糖肽的高通量结构分析

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摘要

Glycopeptide sequencing by collision-induced dissociation (CID) usually produces an intense glycan cleavage, which represents a major disadvantage for the determination of peptide glycosylation sites. Electron transfer dissociation (ETD) is therefore a valuable option for identification of glycosylation sites since ETD induces N-C alpha bond cleavages of peptide backbone with preservation of glycan attachment [1,2]. However, the alternating use of CID and ETD for the same precursor ion and/or the consecutive application of CID/ETD may provide sequence information from which the characterization of peptide backbone, glycan moiety and glycosylation site(s) is possible. Although modern ion trap instruments are able perform automated CID/ETD sequencing in autoMS~n, the experiment cannot be carried out entirely in a high-throughput mode [3] because of the spraying system based on either manual sample load in nanoelectrospray (nanoESI) capillaries or syringe pump infusion.
机译：通过碰撞诱导的解离（CID）的糖肽测序通常产生强烈的聚糖切割，这代表了肽糖基化位点的测定的主要缺点。因此，电子转移解离（ETD）是鉴定糖基化位点的有价值的选择，因为ETD诱导肽骨架的N-Cα键裂解，以保存聚糖附着[1,2]。然而，用于同一前体离子和/或CID / ETD的连续应用的CID和ETD的交替使用可以提供肽主链，甘油部分和糖基化位点的表征的序列信息。虽然现代离子陷阱仪器能够以自动执行自动CID / ETD测序，但实验不能完全以高通量模式[3]进行，因为喷涂系统基于纳米电池普雷（纳米纳米）的手动样品负荷毛细血管或注射器泵输注。

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