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首页> 外文会议>Conference on sensing technologies for global health, military medicine, disaster response, and environmental monitoring >Amplification-free point of care immunosensor for detecting type V collagen at a concentration level of ng/ml
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Amplification-free point of care immunosensor for detecting type V collagen at a concentration level of ng/ml

机译：免疫传感器的免疫传感器，用于检测Ng / ml浓度水平的型胶质胶原蛋白

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Point-of-care testing (POCT) is applicable in the immediate vicinity of the patient, where timely diagnosis or prognostic information could help doctors decide the following treatment. Among types of developed POCT, gold nanoparticle based lateral flow strip technology provides advantages such as simple operation, cost-effectiveness, and a user-friendly platform. Therefore, this type of POCT is most likely to be used in battlefields and developing countries. However, conventional lateral flow strips suffer from low detection limits. Although enzyme-linked amplification was demonstrated to improve the detection limit and sensitivity by stronger visible lines or by permitting electrochemical analytical instrumentation, the enzyme labels have potential to cause interference with other enzymes in our body fluids. To eliminate this limitation, we developed an amplification-free gold nanoparticle-based immunosensor applied for detecting collagen type V, which is produced or released abnormally during rejection of lung transplants and sulfur mustard exposure. By using suitable blocking protein to stabilize gold nanoparticles as the reporter probe, a low detection limit of ng/ml was achieved. This strategy is a promising platform for clinical POCT, with potential applications in military or disaster response.
机译：护理点测试（POCT）适用于患者的直接附近，在那里及时诊断或预后信息可以帮助医生决定以下治疗。在发达的POCT类型中，基于金纳米粒子的横向流动条技术提供了简单的操作，成本效益和用户友好平台等优点。因此，这种类型的POCT最有可能在战场和发展中国家使用。然而，传统的横向流动条患有低检测限。虽然酶联扩增被证明通过更强的可见线或通过允许电化学分析仪器来改善检测极限和敏感性，但酶标记具有可能导致对我们体液中其他酶的干扰。为了消除这种限制，我们开发了一种用于检测胶原型V的基于基于纳米颗粒的免疫传感器，其在抑制肺移植和硫芥末暴露期间产生或释放。通过使用合适的阻断蛋白稳定金纳米颗粒作为报告探针，实现了Ng / ml的低检测限。该策略是临床POCT的有希望平台，具有军事或灾害反应的潜在应用。

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