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首页> 外文会议>Sensing technologies for global health, military medicine, disaster response, and environmental monitoring; and Biometric technology for human identification VIII >Amplification-free point of care immunosensor for detecting type V collagen at a concentration level of ng/ml
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Amplification-free point of care immunosensor for detecting type V collagen at a concentration level of ng/ml

机译：用于检测V型胶原蛋白浓度为ng / ml的免扩增护理点免疫传感器

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Point-of-care testing (POCT) is applicable in the immediate vicinity of the patient, where timely diagnosis or prognostic information could help doctors decide the following treatment. Among types of developed POCT, gold nanoparticle based lateral flow strip technology provides advantages such as simple operation, cost-effectiveness, and a user-friendly platform. Therefore, this type of POCT is most likely to be used in battlefields and developing countries. However, conventional lateral flow strips suffer from low detection limits. Although enzyme-linked amplification was demonstrated to improve the detection limit and sensitivity by stronger visible lines or by permitting electrochemical analytical instrumentation, the enzyme labels have potential to cause interference with other enzymes in our body fluids. To eliminate this limitation, we developed an amplification-free gold nanoparticle-based immunosensor applied for detecting collagen type V, which is produced or released abnormally during rejection of lung transplants and sulfur mustard exposure. By using suitable blocking protein to stabilize gold nanoparticles as the reporter probe, a low detection limit of ng/ml was achieved. This strategy is a promising platform for clinical POCT, with potential applications in military or disaster response.
机译：即时检验（POCT）适用于患者附近，及时诊断或提供预后信息可帮助医生确定以下治疗方案。在已开发的POCT类型中，基于金纳米颗粒的侧流剥离技术具有操作简便，成本效益高和用户友好平台等优点。因此，这种POCT最有可能在战场和发展中国家使用。然而，常规的横向流动条具有低检测极限。尽管已证明酶联扩增可通过更强的可见线或允许使用电化学分析仪器来提高检测限和灵敏度，但酶标记可能会干扰我们体液中的其他酶。为了消除此限制，我们开发了一种无扩增的基于金纳米粒子的免疫传感器，用于检测V型胶原蛋白，该胶原蛋白在肺移植排斥和硫芥子暴露期间异常产生或释放。通过使用合适的封闭蛋白来稳定金纳米颗粒作为报告探针，可以实现ng / ml的低检测限。该策略是临床POCT的有前途的平台，在军事或灾难响应中具有潜在的应用。

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