• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>SPIE Conference on Biomedical Applications in Molecular, Structural, and Functional Imaging >Implementation and assessment of an animal management system forsmall-animal micro-CT / micro-SPECT imaging
【24h】

Implementation and assessment of an animal management system forsmall-animal micro-CT / micro-SPECT imaging

机译：动物管理系统的实施与评估福尔马尔 - 动物微型CT /微观图形成像

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Advances in laboratory imaging systems for CT, SPECT, MRI, and PET facilitate routine micro-imaging during pre-clinical investigations. Challenges still arise when dealing with immune-compromised animals, biohazardous agents, and multi-modality imaging. These challenges can be overcome with an appropriate animal management system (AMS), with the capability for supporting and monitoring a rat or mouse during micro-imaging. We report the implementation and assessment of a new AMS system for mice (PRA-3000 / AHS-2750, ASI Instruments, Warren MI), designed to be compatible with a commercial micro-CT / micro-SPECT imaging system (eXplore speCZT, GE Healthcare, London ON). The AMS was assessed under the following criteria: 1) compatibility with the imaging system (i.e. artifact generation, geometric dimensions); 2) compatibility with live animals (i.e. positioning, temperature regulation, anesthetic supply); 3) monitoring capabilities (i.e. rectal temperature, respiratory and cardiac monitoring); 4) stability of co-registration; and 5) containment. Micro-CT scans performed using a standardized live-animal protocol (90 kVp, 40 mA, 900 views, 16 ms per view) exhibited low noise (±19 HU) and acceptable artifact from high-density components within the AMS (e.g. ECG pad contacts). Live mice were imaged repeatedly (with removal and replacement of the AMS) and spatial registration was found to be stable to within ±0.07 mm. All animals tolerated enclosure within the AMS for extended periods (i.e. > one hour) without distress, based on continuous recordings of rectal temperature, ECG waveform and respiratory rate. A sealed AMS system extends the capability of a conventional micro-imaging system to include immune-compromised and biosafety level 2 mouse-imaging protocols.
机译：在临床前调查期间，CT，SPECT，MRI和PET的实验室成像系统的进步促进常规微量成像。在处理免疫损害的动物，生物隐缩剂和多种式成像时仍然出现挑战。可以使用适当的动物管理系统（AMS）来克服这些挑战，其能够在微观成像期间支持和监测大鼠或小鼠的能力。我们举报了对小鼠的新AMS系统的实施和评估（PRA-3000 / AHS-2750，ASI仪器，沃伦MI），旨在与商业微型CT / Micro-Spect成像系统兼容（探索Speczt，GE医疗保健，伦敦）。在以下标准下评估AMS：1）与成像系统的兼容性（即工件生成，几何尺寸）; 2）与活动物的兼容性（即定位，温度调节，麻醉供应）; 3）监测能力（即直肠温度，呼吸和心脏监测）; 4）共同登记的稳定性; 5）遏制。使用标准化的实际动物协议（90 kVP，40 mA，900次观看，16ms /次）进行的微型CT扫描表现出低噪声（±19胡）和来自AMS内的高密度部件的可接受的伪影（例如ECG垫联系人）。屡次成像活小鼠（伴随AMS的去除和更换），并且发现空间登记在±0.07mm以内稳定。所有动物在AMS中耐受封闭的封闭（即1小时）没有痛苦，基于直肠温度，心电图波形和呼吸率的连续记录。密封的AMS系统延伸了传统微成像系统的能力，包括免疫损害和生物安全级别2小鼠成像协议。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号