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In Vitro Establishment and Proliferation of Red Currant Cultivar 'Detvan'

机译：红醋栗品种“DEDVAN”的体外建立和增殖

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The objective of this study was to develop in vitro multiplication protocol for use with commercially important red currant cultivar 'Detvan'. Initial explants were successfully established in vitro using mercuric chloride in a concentration of 0.15% as a sterilization solution. The overall rate of contamination was 9.7% only. Most of the uncontaminated explants produced new plants in the form of rosettes with short internodes and small leaves after four weeks in culture. To evaluate the shoot multiplication two different mineral media MS and WPM containing different concentrations of growth regulators were employed. Neither of the used media promoted markedly in vitro shoot formation. On MS medium, the number of newly formed shoots in particular subcultures varied between 1.5 and 2.5. Generally, the highest multiplication rate (2.5) was obtained for 'Detvan' on MS medium with 2 mg L~(-1) BAP, 0.5 mg L~(-1) IBA and 0.1 mg L~(-1) GA_3. On MS medium, it was possible to maintain actively growing in vitro shoots during the first six to eight subcultures only. After a total culture period of about 9 months, in vitro plants stopped multiplication and subsequently died out. WPM medium proved to be unsuitable for the proliferation of tested cultivar. Explants on this medium did not multiply and developed symptoms of yellowing or chlorosis.
机译：本研究的目的是开发体外乘法协议，以便与商业上重要的红醋栗品种“DETVAN”一起使用。最初的外植体在体外使用浓度为0.15％作为灭菌溶液的汞氯化物成功建立。整体污染率仅为9.7％。大多数未受污染的外植体以葫芦形式产生新的植物，在培养4周后，短暂的间隙和小叶子。为了评价拍摄倍增，采用两种不同的矿物介质MS和含有不同浓度的生长调节剂的WPM。二手媒体均未明显促进体外芽形成。在MS培养基上，特别是特定推子的新形成的枝条的数量在1.5和2.5之间变化。通常，在MS培养基上获得最高的倍增速率（2.5），以2mg L〜（-1）Bap，0.5mg L〜（-1）IBA和0.1mg L〜（-1）Ga_3。在MS培养基上，只有在前六到八个潜水管中，就可以在体外芽中积极生长。经过大约9个月的总培养期后，体外植物停止繁殖，随后死亡。 WPM培养基证明不适合测试品种的增殖。在这种培养基上的外植体没有繁殖和发育泛黄或萎黄的症状。

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