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首页> 外文会议>International Conference on Energy, Environment and Sustainable Development >Application of carboxymethyl chitosan on Adsorbent detoxification of paralytic shellfish poisoning toxins from Chlamys nobilis
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Application of carboxymethyl chitosan on Adsorbent detoxification of paralytic shellfish poisoning toxins from Chlamys nobilis

机译：Carboxymethyl壳聚糖在Chllmys Nobilis中吸附贝类中毒毒素吸附解毒的应用

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This study investigates the effectiveness of adding carboxymethyl chitosan during steam cooking as an adsorbent for detoxifying paralytic shellfish poisoning toxins (PSP toxins) in the scallop Chlamys nobilis. Toxin analysis using a mouse bioassay test and hydrophilic interaction liquid chromatography-tandem mass spectrometry method (LC-MS) showed that most of the PSP toxins (>80%) were contained in the visceral compartment of the raw scallops. Overall, 82.2Mu/100g of PSP toxins were released from scallop tissues during steaming. The toxicity of the soup steamed with 0.1%, 0.3%, 0.6%, 1.0% and 1.5% carboxymethyl chitosan decreased the toxin content by 35.6%, 44%, 48.4%, 53.3% and 56.9% (p<0.005), respectively. The relative PSP toxin content in the raw adductor muscle was comparable to those after steaming or steaming with carboxymethyl chitosan (p > 0.05).The PSP toxin concentrations in the adductor muscles, gills + mantle and visceral compartments steamed with 0.1%, 0.3%, 0.6%, 1% and 1.5% carboxymethyl chitosan were not significantly different from those in the corresponding raw samples. The LC-MS analysis showed that the adsorbance of carboxymethyl chitosan for each of the PSP toxins was positive correlation with concentration, although the uptake efficiency of each toxin was different. The reduction in toxin content of all analyzed toxins reached 45.6%, 30.2%, 44.5%, 55.9% and 37.6% under the corresponding carboxymethyl chitosan concentrationsis.
机译：本研究研究了在蒸汽烹饪过程中加入羧甲基壳聚糖作为吸附剂在扇贝Chllmys Nobilis中解毒的吸附剂中添加羧甲基壳聚糖作为吸附剂。使用小鼠生物测定试验和亲水性相互作用液相色谱 - 串联质谱法（LC-MS）的毒素分析表明，大多数PSP毒素（> 80％）含有在原料扇贝的内脏隔室中。总体而言，在蒸汽期间，82.2Mu / 100g PSP毒素从扇贝组织中释放出来。汤的毒性以0.1％，0.3％，0.6％，1.0％和1.5％羧甲基壳聚糖分别降低毒素含量35.6％，44％，48.4％，53.3％和56.9％（P <0.005）。 Raw接合肌中的相对PSP毒素含量与蒸汽或用羧甲基壳聚糖蒸发（P> 0.05）进行蒸汽的相对。PSP毒素浓度在内接和肌肉，鳃+地幔和内脏隔室蒸，0.1％，0.3％， 0.6％，1％和1.5％羧甲基壳聚糖与相应的原样中没有显着差异。 LC-MS分析表明，对于每种PSP毒素的羧甲基壳聚糖的吸附与浓度为正相关，尽管每种毒素的摄取效果不同。在相应的羧甲基壳聚糖浓度下，所有分析的毒素的毒素含量的降低达到45.6％，30.2％，44.5％，55.9％和37.6％。

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