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The Efficient Wool Keratin Dissolution Based on New Reductant

机译：基于新还原剂的有效羊毛角蛋白溶解

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The dissolution of the wool keratin has a history of several decades, but the effective dissolution method has not been found yet. In this article, a novel reducing agent - TCEP (Tris (2-carboxyethyl) phosphine) was used, which has a strong ability for selective reduction of disulfide bonds. With a joint use of sodium bisulfite, the wool dissolution has a great advantage in wool processing compared with previous methods; Through amino acid analysis, it was found that the content of the cysteine decreased significantly when using the TCEP as reducing agent, and the other amino acids did not change significantly, indicating that TCEP has high selectivity for reduction of disulfide bonds, and plays a significant role to maintain the molecular weight of keratin. The efficiency of dissolution was greatly improved; dissolution time was 4h, dissolution rate was 93.6%, keratin concentration was 10%, the yield of keratin with the molecular weight more than 3500 and more than 8000 were 80% and 72% respectively. By using SDS-PAGE, we can get that the main molecular weight of the keratin was 14000-20000.
机译：羊毛角蛋白的溶解有几十年的历史，但尚未发现有效的溶解方法。在本文中，使用新型还原剂 - TCEP（Tris（2-羧乙基）膦），其具有强的选择性减少二硫键的能力。随着亚硫酸氢钠的关节使用，与先前的方法相比，羊毛溶解在羊毛加工中具有很大的优势;通过氨基酸分析，发现使用TCEP作为还原剂时半胱氨酸的含量显着下降，并且其他氨基酸不会显着变化，表明TCEP具有高选择性，用于减少二硫键，并发挥重要选择性保持角蛋白的分子量的作用。溶解效率大大提高;溶解时间为4H，溶出速率为93.6％，角蛋白浓度为10％，分子量大于3500以上的角蛋白的产率分别为80％和72％。通过使用SDS-PAGE，我们可以让角蛋白的主要分子量为14000-20000。

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