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首页> 外文会议>UKM FST Postgraduate Colloquium >Analysis of The Relationship Between Chalcone Isomerase Gene Expression Level and Rutin Production in Ficus deltoidea var. deltoidea and F. deltoidea var. angustifolia
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Analysis of The Relationship Between Chalcone Isomerase Gene Expression Level and Rutin Production in Ficus deltoidea var. deltoidea and F. deltoidea var. angustifolia

机译：榕酮异构酶基因表达水平与Ficus Deltoidea VAR的芦丁产生的关系分析。 Deltoidea和F. deltoidea var。 angustifolia

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摘要

Ficus deltoidea (moraceae) is a herbal plant with medicinal values. Previous studies reported that the F. deltoidea contains a high level of bioactive compounds such as flavonoids. A cDNA encodes for chalcone isomerase was identified from F. deltoidea, designated as FdCHI, which involved in the isomerization of naringenin chalcone to naringenin. Naringenin is a key branch point for the synthesis of rutin, which is believed involved in defense mechanism in the plant. Therefore, we hypothesized that there might be a direct relationship between FdCHI expression level and rutin production in leaves of F. deltoidea var. deltoidea (FDD) and F. deltoidea var. angustifolia (FDA). Our result showed that expression level of FdCHI in leaves FDD was greater than FDA. Analysis of High Performance Liquid Chromatography (HPLC) revealed that rutin was only detected in FDA leaves. Based on the results between FdCHI expression and rutin production, this study concluded that there is no relationship between FdCHI expression and rutin production in leaves of FDA and FDD.
机译：薜deltoidea（桑科）是一种草本植物具有药用价值。先前的研究报道，F. deltoidea包含如类黄酮的生物活性化合物的较高水平。的cDNA编码的查耳酮异构酶是从F. deltoidea，指定为FdCHI，这涉及在柚皮素查耳酮向柚皮素的异构化鉴定。柚皮素是一关键分支点芦丁的合成，这被认为参与了植物防御机制。因此，我们推测有可能是在F. deltoidea变种的叶FdCHI表达水平和芦丁的生产有直接的关系。 deltoidea（FDD）和F. deltoidea变种。沙枣（FDA）。我们的研究结果表明FdCHI的表达水平在叶FDD比FDA更大。高效液相色谱法（HPLC）分析表明，芦丁仅在FDA叶检测。基于FdCHI表达和芦丁生产之间的结果，这项研究的结论是，在FDA和FDD的叶FdCHI表达和芦丁的生产没有任何关系。

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