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Cytotoxic and Genotoxic Potency Screening of Two Pesticides on Allium cepa L.

机译:在葱属CEPA L中的两种农药的细胞毒性和遗传毒性效力筛选。

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The cyto- and genotoxicological potency of diclofop-methyl (herbicide) and lindane (insecticide) was screened using Allium cepa L. test. Onion roots were exposed for 24 h to three doses, representing 1/4, 1/2 and EC_(50) concentrations of each pesticide. Some microscopic endpoints as: mitotic and phase indexes, interphase nuclear volume and chromosomal aberration rate and types were evaluated. The results showed obvious concentration-dependence. Mitotic index decreased to 45-59% and 24-37%, while interphase nuclear volume was reduced to 49-54% and 36-42%, for diclofop-methyl and lindane respectively. Abnormal cell's frequency was considerably increased, too. Physiological and clastogenic types of chromosomal aberrations, as: stickiness, c-mitosis, c-anaphase, fragment and bridges, multipolarity and laggard chromosomes were observed in all corresponding concentrations. Data of this study showed that both analyzed pesticides (commonly used for decades in Albanian agriculture) can potentially induce cyto/genotoxic effect on crops and ultimately damage biota and human health.
机译:禾草灵(除草剂)和林丹(杀虫剂)的cyto-和genotoxicological效力使用洋葱L.试验筛选。葱根暴露24小时,以三种剂量,表示每个农药的1/4,1/2和EC_(50)的浓度。一些微观终点为:有丝分裂和相位指标,相间核体积和染色体畸变率和类型进行了评价。结果表现出明显的浓度依赖性。有丝分裂指数降低到45-59%和24-37%,而相间核体积分别减少至49-54%和36-42%,对于禾草灵和林丹。异常细胞的频率大大增加了。生理和诱裂类型的染色体畸变,如:粘性,C-有丝分裂,C-后期,片段和桥梁,多极化和落后的染色体中的所有相应的浓度中观察到。本研究的数据表明,两种分析杀虫剂(通常使用了几十年中阿尔巴尼亚人农业)可以潜在地诱导对作物细胞学/基因毒性作用,并最终损坏生物和人类健康。

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