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Isolation, Identification, and Characterization of an Organophosphorous Pesticide Degrading Bacterium, Enterobacter ludwigii M2

机译:有机磷农药降解细菌的分离,鉴定和表征,肠杆菌Ludwigii M2

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Strain M2 which can degrade organophosphorus pesticide residues was isolated from the soil. It could tolerate concentrations up to 500mg/L of methyl parathion, 500mg/L of chlorpyrifos and 1000mg/L of dimethoate in mineral salt medium containing 0.5% sucrose, In the soil test, strain M2 could degrade 93% of methyl parathion in the soil within 32 days, 71% of chlorpyrifos within 54 days. The degradation rate of strain M2 is much greater than that of natural microorganisms, soil adsorption and the capacity of natural degradation. Strain M2 was identified as a pesticide-degrading strain of Enterobacter ludwigii according to phenotype, biochemical properties and 16S rDNA sequence analysis. The type strain was M2 deposited in China General Microbiological Culture Collection Center on June 8, 2009 and the collection number is CGMCC NO.3092.
机译:从土壤中分离出可以降解有机磷农药残留的菌株M2。它可以耐受高达500mg / L甲基硫磷的浓度,500mg / L含有0.5%蔗糖的矿物盐培养基中的500mg / L紫外酸盐和1000mg / L二甲酯,在土壤试验中,菌株M2可降解土壤中的93%的甲基硫磷在32天内,在54天内含有71%的氯吡啶。菌株M2的降解速率远大于天然微生物,土壤吸附和自然降解能力的降解率。根据表型,生物化学特性和16S rDNA序列分析,将菌株M2鉴定为肠杆菌肠道杆菌的农药降解菌株。 2009年6月8日在中国沉积的菌株为M2,收集号为CGMCC No.3092。

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