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首页> 外文会议>International Conference on Mathematics and Natural Sciences >Isolation, Purification and Characterization of β-amylase from Dioscorea hispida Dennst
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Isolation, Purification and Characterization of β-amylase from Dioscorea hispida Dennst

机译：二磷酸稻田腹腔β-淀粉酶的分离，纯化和表征

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β-amylase (E.C 3.2.1.2) is an enzyme commonly found in plants and bacteria. The enzyme is an exo-acting carbohydrolase which hydrolyzes α-1.4-glucosidic linkages of starch, removing maltose units from the non-reducing end of the polysaccharide chain, producing β-maltose and β-limit dextrin as the final product. β-amylase is widely distributed in the higher plants such as sweet potato. Besides the use in starch hydrolysis, starch-converting enzymes are also used in a number of other industrial applications, such as laundry and porcelain detergents or as anti-stalling agents in baking. This enzyme was extracted from Dioscorea hispida Dennst in 0.05 M acetate buffer pH 4.8 and followed by ammonium sulfate fractionation at cold temperature (10°C). Ammonium sulfate fractionation was shared into fraction of 0-60%, 60-70%, 70-80% and 80-100%. The fraction containing high of specific activity (determined by Somogyi-Nelson and Lowry methods) was futher purified by dialysis. Fraction with high enzyme activity of β-amylase were fraction 60-70% and 70-80%, with specific activity of Dioscorea hispida Dennst were 1.32 and 1.55 mg sugar.mg protein~(-1).minute~(-1), whereas specific activity of crude extract enzyme was 0.21 mg sugar.mg protein-1.minute-1. After purified with dialysis, fraction with high enzyme activity of β-amylase were fraction of 60-70% and 70-80%, with specific activity of Dioscorea hispida Dennst was 2.72 and 4.24 mg sugar.mg protein~(-1).minute~(-1). The purified Dioscorea hispida Dennst β-amylase from dialysis showed increasing in spesific activity the crude enzyme as much as 24 folds. The characterization of enzyme showed that Dioscorea hispida Dennst derived enzyme had optimum pH of 5.5 and temperature of 70°C. The kinetic parameters of purified Dioscorea hispida Dennst β-amylase showed that the (K_M)~(app), (V_(max))~(app) value and Hill constant were 0.0211 mg/ml, 9.63 mg sugar.minute~(-1) and 1.34, respectively.
机译：β-淀粉酶（例如3.2.1.2）是一种常见于植物和细菌的酶。该酶是一种外氧化物碳水化合物，其水解淀粉的α-1.4-葡糖苷键，从多糖链的非还原端除去麦芽糖单元，产生β-麦芽糖和β-极限糊精作为最终产物。 β-淀粉酶广泛分布在甘薯等高等植物中。除了在淀粉水解中的用途外，淀粉转化酶也用于许多其他工业应用，例如衣物和瓷洗涤剂或烘焙中的防停滞剂。将该酶从二十氯乙酸乙酸盐垫中提取，在0.05M乙酸盐pH 4.8中，然后在寒冷温度（10℃）下硫酸铵分馏。硫酸铵分馏分为0-60％，60-70％，70-80％和80-100％的分数。含有高特异性活性的级分（由Somogyi-Nelson和Lowry方法确定）通过透析纯化。 β-淀粉酶高酶活性的级分60-70％和70-80％，具有紫外线幼虫的特异性活性为1.32和1.55mg糖。蛋白〜（-1）.minute〜（-1），虽然粗提取物酶的比活性为0.21mg糖。蛋白-1。用透析纯化后，β-淀粉酶的高酶活性的级分为60-70％和70-80％，具有紫外线的比例的紫外线Dennst的特异性活性为2.72和4.24mg糖.mg蛋白〜（-1）。物质〜（-1）。来自透析的纯化的Dioscorea HispidaDennstβ-淀粉酶显示出在粗酶的情况下增加，粗酶尽可能多为24倍。酶的表征表明，二磷酸钠脱达衍生的酶的最佳pH为5.5和温度为70℃。纯化的二磷酸幼虫的动力学参数HispidaDennstβ-淀粉酶显示（k_m）〜（app），（v_（max））〜（app）值和山常数为0.0211mg / ml，9.63mg糖..（ - 1）分别为1.34。

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