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首页> 外文会议>Conference on energy-based treatment of tissue and assessment VIII >Cells Exposed to Nanosecond Electrical Pulses Exhibit Genetic Biomarkers of Mechanical Stress
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Cells Exposed to Nanosecond Electrical Pulses Exhibit Genetic Biomarkers of Mechanical Stress

机译：暴露于纳秒电脉冲的细胞表现出机械应力的遗传生物标志物

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Exposures of cells to very short (＜1 μs) electric pulses in the megavolt/meter range have been shown to cause disruption of the plasma membrane. This disruption is often characterized by the formation of numerous small pores (＜2 nm in diameter) in the plasma membrane that last for several minutes, allowing the flow of ions into the cell. These small pores are called nanopores and the resulting damage to the plasma membrane is referred to as nanoporation. Nanosecond electrical pulse (nsEP) exposure can impart many different stressors on a cell, including electrical, electro-chemical, and mechanical stress. Thus, nsEP exposure is not a "clean" insult, making determination of the mechanism of nanoporation quite difficult. We hypothesize that nsEP exposure creates acoustic shock waves capable of causing nanoporation. Microarray analysis of primary adult human dermal fibroblasts (HDFa) exposed to nsEP, indicated several genes associated with mechanical stress were selectively upregulated 4 h post exposure. The idea that nanoporation is caused by external mechanical force from acoustic shock waves has, to our knowledge, not been investigated. This work will critically challenge the existing paradigm that nanoporation is caused solely by an electric-field driven event and could provide the basis for a plausible explanation for electroporation.
机译：已经显示了MegaVolt /仪表范围内的细胞暴露于非常短的（<1μs）电脉冲，导致质膜中断。这种破坏通常是在持续几分钟的血浆膜中形成许多小孔（直径直径）的形成，使得离子流入细胞中。这些小孔称为纳米孔，并将所得对质膜的损伤称为纳米孔。纳秒电脉冲（NSEP）曝光可以在电池上赋予许多不同的压力，包括电气，电化学和机械应力。因此，NSEP曝光不是“干净”的侮辱，制定纳米孔的机制非常困难。我们假设NSEP暴露产生能够引起纳米孔的声学冲击波。小阵列分析初级成人人的皮肤成纤维细胞（HDFA）暴露于NSEPP，表明了与机械应激相关的几个基因，选择性地上调了4小时暴露。纳米流体是由声学冲击波引起的纳米流体引起的想法，我们的知识没有被调查。这项工作将严重挑战现有的范式，即纳米流体仅通过电场驱动事件引起的，并且可以为电穿孔的合理解释提供基础。

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