• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Conference on imaging, manipulation, and analysis of biomolecules, cells, and tissues XIII >Protein patterning: a comparison of direct spotting versus microcontact printing
【24h】

Protein patterning: a comparison of direct spotting versus microcontact printing

机译：蛋白质图案化：直接斑点与微接触印刷的比较

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Protein microarrays are used various research areas including drug discovery, diagnosis, and analysis of protein-ligand interactions. Their efficacy depends on a well-defined pattern of immobilized proteins that also have retained their bioactivity. Protein microarrays are classically fabricated using the robotic spotting drop method ("pin printing"), which can lead to spots with uneven protein concentration within the spotted area, leading to difficult to quantify readings. Among the alternative techniques, microcontact printing (μCP) with a poly(dimethylsiloxane) (PDMS) stamp appears to deliver more defined protein patterns on surfaces, while maintaining bioactivity for a wide range of proteins. Here we have quantitatively compared the distribution of fluorescently labeled proteins deposited using direct pipetting, pin printing and uCP printing with flat stamps onto various functionalized glass surfaces of different contact angles through fluorescent microscopy. The uniformity of the deposited protein spots across deposition techniques was also qualitatively analyzed. It was found that with the use of either the direct pipetting or pin printing techniques that protein concentration on surfaces varied largely across surfaces with different contact angles, whereas adsorption did not vary significantly when using the uCP printing Furthermore, when uCP printing was performed with flat relief structures the spot inhomogeneity was lower than when classical methods were used, and even less so when a pyramid relief structure was used. This suggests that uCP printing with pyramid relief structures could produce protein patterns on various surfaces and with increased spot uniformity to enable more reliable protein microarrays.
机译：使用蛋白质微阵列使用各种研究领域，包括药物发现，诊断和蛋白质 - 配体相互作用的分析。它们的功效取决于固定化蛋白质的明确定义的模式，该蛋白质也保留了它们的生物活性。使用机器人斑点下降方法（“销印刷”）经典制造蛋白质微阵列，这可能导致斑点区域内的蛋白质浓度不均匀，导致难以量化读数。在替代技术中，具有聚（二甲基硅氧烷）（PDMS）印模的微接触印刷（μCP）似乎在表面上递送更明确的蛋白质图案，同时保持各种蛋白质的生物活性。在这里，我们已经定量地比较了使用直接移液，销印刷和UCP打印的荧光标记的蛋白质的分布，通过荧光显微镜通过荧光显微镜在不同接触角的各种官能化玻璃表面上。还定性地分析了穿过沉积技术的沉积蛋白质斑点的均匀性。发现使用直接移液或引脚印刷技术，在表面上使用不同的接触角的表面越差而在表面上变化很大，而当使用平面的UCP印刷时，吸附在使用UCP印刷时不会显着变化浮雕结构点不均匀性低于使用经典方法时，甚至更少时，当使用金字塔浮雕结构时。这表明具有金字塔浮雕结构的UCP打印可以在各种表面上产生蛋白质图案，并且具有增加的点均匀性，以实现更可靠的蛋白质微阵列。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号