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首页> 外文会议>International Conference on Biological Science >Metabolic Profiling of Endophytic Bacteria from Purwoceng (Pimpinella pruatjan Molkend) Root and Antibacterial Activity against Staphylococcus aureus and Pseudomonas aeruginosa
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Metabolic Profiling of Endophytic Bacteria from Purwoceng (Pimpinella pruatjan Molkend) Root and Antibacterial Activity against Staphylococcus aureus and Pseudomonas aeruginosa

机译：来自Puriwoceng（Pimpinella Pruatjan Molkend）根系和抗菌活性对葡萄球菌和假单胞菌的抗菌活性的代谢谱

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Recent study on antibiotic produced by endophytic bacteria become emerging to fight against the increasing of drug-resistant bacteria. Twenty eight isolates of endophytic bacteria from Purwoceng (Pimpinella pruatjan Molkend) have been screened for their potential in preventing growth of pathogens, including Staphylococcus aureus and Pseudomonas aeruginosa. In the present study four isolates (GP11, GP12, RP6 and DG1) and another three isolates (GP2, GP14 and DG1) showed the activity to prevent the growth of S. aureus and P. aeruginosa ATCC 27853, respectively. Bioactive compounds were extracted from culture broth using ethyl acetate, followed by Thin layer chromatography (TLC) for metabolic profiling to identify the chemical class of compounds. Taxonomic status of the isolates was determined by BLAST analysis based on 16S rRNA sequences. The result of inhibition zone from 500 mg · mL~(-1) crude extract DG1, GP11, GP12 and RP6 against S. aureus respectively are 0.78 cm, 1.18 cm, 0.95 cm and 1.2 cm, whereas for 25 mg · mL~(-1) crude extract DG1, GP2 and GP14 against P. aeruginosa respectively are 0.144 cm, 0.151 em and 0.095 cm. TLC assay showed that endophytic bacteria produce alkaloid compound and from the BLAST analysis based on 16S rRNA sequences isolates closely related to Bacillus subtilis (GP11, GP12, GP14 and DG1), Bacillus cereus (RP6) and Enterococcus faecalis (GP2).
机译：最近对内生细菌产生的抗生素的研究变得抗抗药性细菌的增加。已经筛查了来自Puriceng（PimpinellaPruatjan Molkend）的二十八个分离物的内生细菌，用于预防病原体生长的潜力，包括金黄色葡萄球菌和假单胞菌铜绿假单胞菌。在本研究中，四个分离株（GP11，GP12，RP6和DG1）和另外三个分离株（GP2，GP14和DG1）显示了防止S.UUREUS和P.铜绿假单胞菌ATCC 27853的生长。使用乙酸乙酯从培养液中萃取生物活性化合物，然后用薄层色谱（TLC）用于代谢分析以鉴定化合物的化学类别。基于16S rRNA序列，通过BLAST分析确定分离物的分类状态。抑制区从500mg·mL〜（-1）粗提取物DG1，GP11，GP12和RP6分别为0.78cm，1.18cm，0.95cm和1.2cm，而25 mg·ml〜（ -1）粗提取物DG1，GP2和GP14针对P.铜绿假单胞菌分别为0.144cm，0.151米和0.095厘米。 TLC测定表明，内生细菌产生了生物碱化合物，并基于16S rRNA序列分离物与枯草芽孢杆菌（GP11，GP12，GP14和DG1），芽孢杆菌（RP6）和肠球菌粪便（GP2）密切相关的分离物。

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