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The effect of photoperiod on lipid production of mixed culture Arthrospira maxima Setchell et Gardner and microalgae consortium of Glagah isolate

机译:光周期对混合培养的脂质生成的影响arthrospira maxima setchell等加尔德纳和微藻菌的胶原菌分离株

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Microalgae are organisms with huge potential to be a source of biodiesel production. Various types of microalgae have different capacities to produce lipids. Mixed cultures such as Arthrospira maxima and microalgae consortium of Glagah isolate are known to produce lipids higher than single culture. Light is an important factor for microalgae, it was needed as a source of energy in photosynthesis. And photosynthesis was the key to hold metabolic processes in microalgae. Light factors that can affect the phot osynthesis include light intensity, wavelength and duration of radiation (photoperiod). The longer illumination will boost the growth of microalgae cell. In the microalgae industry, especially in indoor light is a factor that takes up a lot of production costs. So the use of light energy should be made as efficient as possible to reduce the cost of production. Therefore, this study aimed to know the duration of radiation that most efficiently promoted cell growth and lipid production of mixed cultures A. maxima and microalgae consortium of Glagah isolate. Variations in the light -dark photoperiod used were 12-12, 16-8, 20-4, and 24-0 hours. The method used to calculate the density of cells was usin g Haemocytometer and for lipid analysis was using the method o f Bligh and Dyer. The results obtained showed that the 16-8 hour light-dark treatments could generate cell density and lipid production with the highest which each value were 5.32 million cell·mL~(-1) and 0.27 mg·mL~(-1), respectively with the percentage of lipid by dryweight was 29.57%. in conclusion, the photoperiod 16 hours of light - 8 hours of dark produce the lipid content of mixed culture of A. maxima and a consortium of microalgae Glagah isolates which reaches 0.27 mg·mL-1. In other words, the production of lipids produced from this mixed culture is 29.57% of its dry weight. The 16 hours of light photoperiod - 8 hours dark produces the highest number of mixed cultures of A. maxima and a microalgae consortium of Glagah isolates. The relationship between photoperiod with other factors, such as light intensity, temperature, and nutrients were needed to obtain an optimal lipid production of A. maxima and the consortium of Glagah isolates.
机译:微藻是具有巨大潜力的生物,成为生物柴油生产的来源。各种类型的微藻具有产生脂质的不同能力。已知混合培养物如arthrospira maxima和微藻聚集体的胶原酸盐分离物,产生高于单一培养物的脂质。光是微藻的一个重要因素,需要作为光合作用中的能量来源。和光合作用是在微藻中保持代谢过程的关键。可以影响光伏替换的光因子包括光强度,波长和辐射持续时间(光周期)。较长的照明将提高微藻细胞的生长。在微藻工业中,特别是在室内灯中是一种占据大量生产成本的因素。因此,应尽可能高效地使用光能以降低生产成本。因此,本研究旨在了解辐射的持续时间,最有效地促进细胞生长和混合培养物的脂质生成A. Maxima和Microalgae联盟的Glagah分离物。使用的光--Dark光周期的变化为12-12,16-8,20-4和24-0小时。用于计算细胞密度的方法是USIN G血慢计,并且用于脂质分析使用该方法O F Bligh和Dyer。得到的结果表明,16-8小时的光暗处理可以产生细胞密度和脂质生产,其中每个值分别为5.32亿细胞·ml·m1(-1)和0.27mg·ml〜(-1)通过脂质的脂质百分比为29.57%。总之,光周期16小时的光 - 8小时的黑暗产生A.Maxima的混合培养物的脂质含量和达到0.27mg·mL-1的微藻胶原分离物的联盟。换句话说,由该混合培养物产生的脂质的生产是其干重的29.57%。 1小时的光光周期 - 8小时黑暗产生A. Maxima和Glagah分离物的微藻半球菌数量最多的混合培养物。需要与其他因素的光电子之间的关系,例如光强度,温度和营养素,以获得A.Maxima和Glagah分离物的联盟的最佳脂质生产。

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