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PCV Measurement for Biomass Assessment in Suspension Culture of Insect Cells

机译:昆虫细胞悬浮培养中生物质评估的PCV测量

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In this study, we present a new approach for biomass assessment in insect cell culture using a disposable microcentrifuge tube, which is composed with an upper chamber for sample loading and a lower 5 μL capillary for cell collection during centrifugation. The height of the cell pellet within the capillary is measured and expressed as the packed cell volume (PCV). PCV method showed an excellent correlation with cell counting measurement, but with lower average of the errors (3.3%) compared to manual cell counting (12.4%). PCV method was shown to be statistically reliable and more precise, rapid. And it also demonstrates the potential use of this method to monitor the changes in insect cell culture that can affect cell size, for example as seen under baculovirus infection.
机译:在这项研究中,我们在使用一次性微量离心管中提出了一种新的昆虫细胞培养物中的生物量评估方法,其与用于样品加载的上腔室和用于离心过程中的细胞收集的下部5μl毛细管组成。测量毛细管内的细胞颗粒的高度并表示为填充细胞体积(PCV)。 PCV方法显示出与细胞计数测量的优异相关性,但与手动细胞计数(12.4%)相比,误差平均值较低(3.3%)。 PCV方法显示出统计上可靠,更精确,快速。并且还证明了这种方法的潜在用途,以监测可以影响细胞尺寸的昆虫细胞培养的变化,例如如杆状病毒感染所示。

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