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首页> 外文会议>International Conference on Biomedical and Biological Engineering >Influence of 660 and 830 Nm Laser Irradiation on Genetic Profile of Extracellular Matrix Proteins in Diabetic Wounded Human Skin Fibroblast Cells
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Influence of 660 and 830 Nm Laser Irradiation on Genetic Profile of Extracellular Matrix Proteins in Diabetic Wounded Human Skin Fibroblast Cells

机译：660和830 nm激光辐射对糖尿病伤口人体皮细胞细胞外基质蛋白遗传谱的影响

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The extracellular matrix (ECM) provides tissue structural integrity and its synthesis plays a crucial role in wound healing. Impaired wound healing ensues following the destruction of the ECM, or the inhibition of its synthesis. An imbalance in ECM synthesis and degradation is seen in patients with diabetes. This has led to the development of novel therapies that aim to decrease ECM destruction and increase its synthesis. Photobiomodulation (PBM) has been shown to speed up the healing of these slow-to-heal wounds, and numerous studies are being conducted to determine the underlying molecular cause. This study aimed to ascertain the effect of laser irradiation at a wavelength of 660 or 830 nm at a dose of 5 J/cm~2 on the genetic expression profile of genes involved in ECM proteins. cDNA was reverse transcribed from total isolated RNA and used in real-time qualitative polymerase chain reaction (qPCR). Genes concerned with the basement membrane, collagen and ECM structural constituents, and ECM protesases and inhibitors were evaluated. Results showed that a similar genetic profile (although not identical) was seen post irradiation. PBM was able to reduce the expression of ECM proteases and increase mRNA levels of ECM proteins. This study fortifies the notion that PBM stimulates cellular activity and can influence ECM matrix synthesis and degradation during wound healing. This study has also shown that PBM is able to stimulate cells at a genetic level.
机译：细胞外基质（ECM）提供组织结构完整性，其合成在伤口愈合中起着至关重要的作用。伤口愈合受损后，在破坏ECM后，或抑制其合成。在糖尿病患者中可以看到ECM合成和降解的不平衡。这导致了新的疗法的发展，该疗法旨在降低ECM破坏并增加其合成。已经证明了光致调节（PBM）加速了这些缓慢愈合伤口的愈合，并且正在进行许多研究以确定潜在的分子原因。该研究旨在确定在ECM蛋白参与的基因的遗传表达谱上以660或830nm的波长为660或830nm的激光照射的影响。 CDNA从总分离的RNA逆转录，并用于实时定性聚合酶链反应（QPCR）。评价有关基底膜，胶原和ECM结构成分和ECM蛋白酶和抑制剂的基因。结果表明，在照射后看到类似的遗传概况（虽然不相同）。 PBM能够降低ECM蛋白酶的表达，增加ECM蛋白的mRNA水平。本研究强化了PBM刺激细胞活性的观念，并可以影响伤口愈合过程中的ECM矩阵合成和降解。该研究还表明，PBM能够在遗传水平下刺激细胞。

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