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首页> 外文学位 >A Tissue Engineered Treatment for Chronic Diabetic Skin Wounds: An Advanced Collagen Wound Matrix Combined with Adipose Derived Stem Cells
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A Tissue Engineered Treatment for Chronic Diabetic Skin Wounds: An Advanced Collagen Wound Matrix Combined with Adipose Derived Stem Cells

机译：慢性糖尿病皮肤伤口的组织工程治疗：先进的胶原蛋白伤口基质与脂肪干细胞相结合。

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摘要

Chronic, non-healing skin wounds are a significant clinical challenge in diabetic patients, and few effective treatments are currently available. Often, after many failed treatments, physicians are forced to resort to amputation of the affected extremity due to the high risk of infection and eventual sepsis. An effective treatment to heal chronic diabetic skin wounds is urgently needed to reduce the risk of infection, eliminate the need for limb amputation, and improve the quality of life of people with diabetes. To address this need, we have developed a tissue-engineered treatment that combines a novel electrochemically deposited type I collagen scaffold, termed the advanced collagen wound matrix (CWM), and human adipose derived stem cells. The CWM is fabricated by an electrochemical deposition method, as opposed to a traditional collagen gelation method, which creates a densely packed, robust collagen matrix that possesses excellent material properties for skin tissue engineering. The CWM possesses high porosity, an appropriate degradation profile, and significantly higher tensile strength than a standard gelated collagen scaffold. The CWM is highly biocompatible and supports cellular growth and proliferation well. To increase the wound healing capabilities of this treatment, the CWM is seeded with human adipose derived stem cells. Adipose derived stem cells (ADSCs) are abundant, easily harvested, and well suited for tissue culture. They also possess inherent wound healing properties, making them ideal for treatment of chronic diabetic skin wounds. To fully investigate this treatment, the CWM with and without the addition of ADSCs is used to treat full thickness excisional skin wounds in a murine model of type 2 diabetes and wound healing is monitored to 21 days. In this model, untreated diabetic skin wounds regenerated low volumes of granulation tissue during the first 7 days of healing, then had no increase in granulation tissue formation past the initial 7 days, highlighting the impaired wound healing capabilities associated with diabetes. Treatment with the CWM alone and the CWM combined with ADSCs, however, consistently stimulated significantly increased volumes of regenerated granulation tissue and formed higher quality tissue than the untreated wounds. We prove that the CWM stimulates healthy tissue regeneration in diabetic skin wounds when used both alone and in combination with adipose derived stem cells. We have developed an excellent treatment option for patients with chronic, non-healing diabetic wounds who may otherwise be forced to consider limb amputation.
机译：慢性，不愈合的皮肤伤口在糖尿病患者中是重大的临床挑战，目前尚无有效的治疗方法。通常，在许多治疗失败后，由于感染和最终败血症的高风险，医生被迫截肢患肢。迫切需要一种有效的治疗慢性糖尿病皮肤伤口的方法，以减少感染的风险，消除肢体截肢的需要并改善糖尿病患者的生活质量。为了满足这一需求，我们开发了一种组织工程疗法，将一种新型的电化学沉积的I型胶原蛋白支架（称为高级胶原蛋白伤口基质（CWM））与人脂肪来源的干细胞结合在一起。与传统的胶原蛋白凝胶化方法相反，CWM是通过电化学沉积方法制造的，传统的胶原蛋白凝胶化方法可产生密集包装的坚固的胶原蛋白基质，该基质具有用于皮肤组织工程的优异材料性能。与标准凝胶胶原支架相比，CWM具有高孔隙率，适当的降解曲线和显着更高的拉伸强度。 CWM具有高度的生物相容性，并很好地支持细胞的生长和增殖。为了增加这种治疗方法的伤口愈合能力，在CWM上接种了人类脂肪来源的干细胞。脂肪来源的干细胞（ADSC）丰富，易于收获，非常适合组织培养。它们还具有固有的伤口愈合特性，使其成为治疗慢性糖尿病皮肤伤口的理想选择。为了全面研究这种治疗方法，在2型糖尿病小鼠模型中，使用添加和不添加ADSC的CWM来治疗全层切除皮肤伤口，并监测伤口愈合至21天。在该模型中，未经治疗的糖尿病皮肤伤口在愈合的前7天中再生出少量肉芽组织，然后在最初的7天中肉芽组织形成没有增加，这突出了与糖尿病相关的伤口愈合能力受损。但是，单独使用CWM和与ADSC组合使用CWM的治疗持续刺激再生肉芽组织的体积显着增加，并且形成的组织质量高于未治疗的伤口。我们证明，当单独或与脂肪干细胞结合使用时，CWM可以刺激糖尿病皮肤伤口中健康的组织再生。我们为患有慢性，无法愈合的糖尿病伤口的患者开发了一种极好的治疗选择，否则这些伤口可能被迫考虑截肢。

著录项

  • 作者

    Edwards, Nicole J.;

  • 作者单位

    The University of Texas at San Antonio.;

  • 授予单位 The University of Texas at San Antonio.;
  • 学科 Biomedical engineering.
  • 学位 Ph.D.
  • 年度 2018
  • 页码 259 p.
  • 总页数 259
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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